We narrowed to 1,322 results for: pET28a

1. pET28_mouse-pro-IL1B

   Plasmid

   #183386

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL1B (Il1b Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_human-Caspase-4_enzymatic-domain

   Plasmid

   #183381

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4 (AA94-377) (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_mouse-pro-IL18

   Plasmid

   #183389

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (Il18 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tUI-HA

   Plasmid

   #122662

   Purpose

   Express tUI-HA protein in E. coli

   Depositor

   Robert Cohen

   Article

   Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   tUI-HA
   

   Use

   Tags

   HA-tag and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Human RNF144A (16-228)

   Plasmid

   #110752

   Purpose

   Bacterial Expression of RNF144A RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144A (RNF144A Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyTag003-mClover3

   Plasmid

   #133453

   Purpose

   Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mClover3
   

   Use

   Tags

   GSGSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human RNF144B (27-236)

   Plasmid

   #110753

   Purpose

   Bacterial Expression of RNF144B RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144B (RNF144B Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETSG

   Plasmid

   #159418

   Purpose

   Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   polyhistidine (poly-His)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG308-His-redFAST

    Plasmid

    #160350

    Purpose

    Expresses His-tagged redFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    His-redFAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG261-His-greenFAST

    Plasmid

    #160349

    Purpose

    Expresses His-tagged greenFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    His-greenFAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDC20

    Plasmid

    #131366

    Purpose

    expression of MEG-3 (aa1-862) in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

    Insert

    meg-3 (aa1-862) (meg-3 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Use

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tISR

    Plasmid

    #122664

    Purpose

    Express tISR protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tISR (OCLM Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pJK667

    Plasmid

    #71690

    Purpose

    Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

    Insert

    acetate kinase (ackA Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK220

    Plasmid

    #71123

    Purpose

    Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    pantothenate kinase
    

    Use

    Tags

    H6

    Expression

    Bacterial

    Mutation

    Arg175 is mutated to a His

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p40 FL

    Plasmid

    #19013

    Depositor

    Michael Yaffe

    Article

    Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pJK511

    Plasmid

    #71591

    Purpose

    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes histidine-222 to glutamine

    Promoter

    T7 promoter

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A2C-∆N

    Plasmid

    #217909

    Purpose

    Expresses bicone gas vesicles in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Ling et al Adv Mater. 2024 Feb 26:e2307106. doi: 10

    Insert

    A2C-∆N
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDL25_Cas9-His Δcys E1207C

    Plasmid

    #206286

    Purpose

    Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDL23_Cas9-His Δcys E945C

    Plasmid

    #206284

    Purpose

    Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDL24_Cas9-His Δcys E1026C

    Plasmid

    #206285

    Purpose

    Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1026C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDL22_Cas9-His Δcys E532C

    Plasmid

    #206283

    Purpose

    Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-28a(+)-His(6)-AtPCO1(wt)

    Plasmid

    #191798

    Purpose

    Full length wt AtPCO1 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO1
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-28a(+)-His(6)-AtPCO2(wt)

    Plasmid

    #191799

    Purpose

    Full length wt AtPCO2 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO2
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-28a(+)-His(6)-AtPCO3(wt)

    Plasmid

    #191800

    Purpose

    Full length wt AtPCO3 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO3
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

    Plasmid

    #183387

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    redesigned Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ANR261ENP, D315H, M318K, T352K

    Promoter

    T7

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FAK C-term

    Plasmid

    #186147

    Purpose

    For expresseion of recombinant FAK c-terminal domain in E. coli

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28_hTLN2-head-LBD

    Plasmid

    #164837

    Purpose

    Expression of human talin2 head mutant

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Lipid binding deficient mutations

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge