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pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR-EASY

Plasmid

#241237

Purpose

CRISPR-Cas9 genome editing

Depositor

Tamara O'Connor

Article

Hossain et al PLoS One. 2024 Mar 14;19(3):e0299513. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PPDK

Plasmid

#64037

Purpose

Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

Depositor

Vern Schramm

Article

Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

Insert

pyruvate phosphate dikinase

Tags

none

Expression

Bacterial

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDSG358

Plasmid

#115599

Purpose

surface-displayed Ag1; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Mutation

See depositor comments below

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-LEU2MX6

Plasmid

#33138

Purpose

gene deletion in S. cerevisiae and S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Insert

LEU2MX6

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAd5-B5

Plasmid

#122555

Purpose

Block 5 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 17998-25062

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDSG290

Plasmid

#115609

Purpose

surface-displayed Nb3, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG288

Plasmid

#115607

Purpose

surface-displayed Ag3, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG312

Plasmid

#115613

Purpose

surface-displayed Nb2, Arabinose-inducible; medium-copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDSG398

Plasmid

#115604

Purpose

surface-displayed Nb3; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Mutation

See depositor comments below

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pYTK-DN3

Plasmid

#180284

Purpose

Spacer cassette (TU2)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL1 - Spacer - ConR2

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pERB254

Plasmid

#67762

Purpose

Tags mitochondria with Halotag and GFP

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

Halo-GFP-Mito

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA-BfuAI-2k

Plasmid

#112915

Purpose

Empty sgRNA expression plasmid

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAd5-B2

Plasmid

#122552

Purpose

Block 2 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 3737-10881

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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mg319

Plasmid

#73367

Purpose

unc-70 tension sensor

Depositor

Miriam Goodman

Article

Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

Insert

unc-70::TSMod

Promoter

unc-70p

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDSG310

Plasmid

#115611

Purpose

surface-displayed Ag2, Arabinose-inducible; medium-copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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