We narrowed to 87,637 results for: MAL

1. mCit(P5f-P13f)3-gs

   Plasmid

   #228080

   Purpose

   Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   mCit, P5f, P13f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TagBFP(P6f)3(P14f)3-gs

   Plasmid

   #228081

   Purpose

   Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   BFP, P6f, P14f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPyPGK-EGFP-TM-IRES-Pac

   Plasmid

   #183604

   Purpose

   Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   Human PDGFRB transmembrane domain and Mouse IgGK …

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Pgk1

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NET31 mRFP-N2 (490)

   Plasmid

   #61984

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

   Insert

   NET31 (TMEM209 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

   Plasmid

   #89608

   Purpose

   Expression of Luciferase in human cells. 3rd generation lentiviral vector

   Depositor

   Fernando Fierro

   Article

   Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MNDU3

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a hMDH2

   Plasmid

   #204230

   Purpose

   Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH2 (MDH2 Human)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459V2.0-HypaCas9-mRuby2

   Plasmid

   #183872

   Purpose

   pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HypaCas9-P2A-mRuby2-T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ-5S-F30-Control

   Plasmid

   #127582

   Purpose

   Expresses 5S-F30-Control rRNA from a mU6 promoter

   Depositor

   David Rueda

   Article

   Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

   Insert

   5S rRNA with F30 folding scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified U6

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. eNme2-C TadCBEd

   Plasmid

   #193841

   Purpose

   Express TadCBEd (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   eNme2-C TadCBEd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176268

    Purpose

    Expression of dCas12f-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCas12f
    

    Use

    Lentiviral

    Tags

    HA-VPR-mCherry

    Expression

    Mammalian

    Mutation

    D326A, D510A

    Promoter

    PGK

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBTetBl-CLIP-STIL

    Plasmid

    #136844

    Purpose

    Mammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-STIL (STIL Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIDTSmart-MbPylRS

    Plasmid

    #217361

    Purpose

    Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. barkeri pyrrolysyl-tRNA synthetase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LbCas12a-2C-NLS in pCSDest

    Plasmid

    #126638

    Purpose

    Expresses LbCas12a-2C-NLS in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ-mgU2-47-Mango II

    Plasmid

    #127586

    Purpose

    Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    mgU2-47 scaRNA with Mango II tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescript-IC3-FLAG-Tev-ZZ

    Plasmid

    #170311

    Purpose

    Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Outer Dynein Arm Intermediate Chain 3
    

    Use

    Tags

    FLAG, ZZ

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCit-APPAPAPPAP-gs

    Plasmid

    #228091

    Purpose

    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, APPAPAPPAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.3_CRKL-WT-V5

    Plasmid

    #196018

    Purpose

    Expresses CRKL WT, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEBTet-SNAP-Centrin3

    Plasmid

    #136806

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.3_CRKL-Y207F-V5

    Plasmid

    #196016

    Purpose

    Expresses CRKL Y207F, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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