Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 21,826 results for: Sis;

Showing: 701 - 720 of 21826 results

  1. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Jen Sheen
    Article
    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis U6 polymerase III promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52255-plasmid-map-sequence-id-80847

    Enlarge

  2. NST1_pOpOn2.1

    Plasmid
    #228065
    Purpose
    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Article
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228065-plasmid-map-sequence-id-456794

    Enlarge

  4. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

    Enlarge

  5. p35S_GFP-EVDinter-GUS

    Plasmid
    #167123
    Purpose
    Plant binary expression vector containing the intron and proximal plyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.
    Depositor
    Arturo Marí-Ordóñez, Olivier Voinnet
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    Use
    Tags
    GUS and mGFP5
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    167123-plasmid-map-sequence-id-331746

    Enlarge

  6. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Use
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

    Enlarge

  10. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

    Enlarge

  12. pAdeasy-CMV-AE-EF1α-R2

    Plasmid
    #194928
    Purpose
    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis
    Depositor
    Limei Wang
    Article
    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.
    Inserts
    AE
    EEF1A1 (EEF1A1 Human)
    R2
    Use
    Adenoviral
    Tags
    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c
    Expression
    Mutation
    a 48 bp linker (Gly3ser)4 was added between the t…
    Promoter
    CMV and EF1a
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    194928-plasmid-map-sequence-id-391107

    Enlarge

  13. pSBBi-GB TCS2

    Plasmid
    #139171
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS2) from Camellia sinensis; the other side contains GFP and blasticidin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    probable caffeine synthase 2 [Camellia sinensis]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139171-plasmid-map-sequence-id-309361

    Enlarge

  15. FoRC.PGa

    Plasmid
    #84584
    Purpose
    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.
    Depositor
    Francesca Pignoni
    Article
    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84584-plasmid-map-sequence-id-166579

    Enlarge

  16. FoRC.PRa

    Plasmid
    #84585
    Purpose
    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.
    Depositor
    Francesca Pignoni
    Article
    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84585-plasmid-map-sequence-id-166585

    Enlarge

  18. FoRC.PRm

    Plasmid
    #84587
    Purpose
    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.
    Depositor
    Francesca Pignoni
    Article
    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84587-plasmid-map-sequence-id-168648

    Enlarge

Showing: 701 - 720 of 21826 results