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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
As2

Plasmid

#123160

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, ParsR-ABS84, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAB085c

Plasmid

#79208

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ptet

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 WT

Plasmid

#27458

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pXWJ109HgAmp30E

Plasmid

#123149

Purpose

Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, PmerT, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-mCherry-N

Plasmid

#210271

Purpose

N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET-CVm-PTEN

Plasmid

#168032

Purpose

Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-PTEN(M265-N329)-mVenus

Use

Tags

Hisx6 and myc

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-shLuc

Plasmid

#73666

Purpose

pSRIEN-RetroQ vector containing control sequence luciferase

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA to Luciferase

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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GAL FLAG25QDProCFPp303

Plasmid

#15584

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Use

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG46QDProGFP p306

Plasmid

#15832

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL 46Q+ProGFPp416

Plasmid

#15581

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL 25Q+ProGFPp416

Plasmid

#15580

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLV-EF1A-LivePAR(R163A)-Hygro

Plasmid

#187609

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

Depositor

Robert Sobol

Article

Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

Insert

LivePAR(R163A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

R163A mutation in the WWE Domain

Promoter

EF1A

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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FusionRed-VASP-5

Plasmid

#56136

Purpose

Localization: Focal Adhesions, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

VASP

Use

Tags

FusionRed

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAB088c

Plasmid

#79213

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ppro1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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GAL 72Q+ProGFPp416

Plasmid

#15582

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
ELC-Fc

Plasmid

#8636

Depositor

Timothy Springer

Article

Manjunath et al J Clin Invest 2001 Sep;108(6):871-8.

Insert

CCL19 (CCL19 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-Tag/M1 S245X

Plasmid

#92366

Purpose

expression of M1 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25Q+ProGFPp303

Plasmid

#15572

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC-delta-IRES-hrGFP

Plasmid

#73414

Purpose

Mammalian expression of mouse PKC-delta and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKC-delta (Prkcd Mouse)

Use

Tags

3xFLAG and IRES-hrGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only