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Golgi-targeted optoPLD

Plasmid

#140058

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pCN38-kimA-yfp

Plasmid

#227635

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

Plasmid

#219555

Purpose

PM-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-lyn10-mCherry-dLOVPLD

Plasmid

#219556

Purpose

PM-targeted catalytically dead LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-mCherry-dLOVPLD

Tags

mCherry

Expression

Mammalian

Mutation

H170A mutation on PLD

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-p18-mCherry-LOVPLD

Plasmid

#219560

Purpose

lysosome-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

p18-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCN38-yfp

Plasmid

#227637

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-eGFP-hTau E14-WPRE

Plasmid

#233042

Purpose

To Express a GFP human Tau E14 fusion protein from a CMV promoter

Depositor

Jonathan Ploski

Insert

eGFP-hTau E14 fusion (MAPT Human, Synthetic)

Use

Lentiviral

Tags

EGFP

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA

Plasmid

#164724

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

Flag

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

Plasmid

#62930

Purpose

pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.1Synapsin and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP

Plasmid

#183916

Purpose

Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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NUMB4-eGFP-M107K

Plasmid

#37805

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 4 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optoPLD

Plasmid

#140114

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Rat SARE-Arc Min Promoter-D2GFP-SV40 3'UTR

Plasmid

#233058

Purpose

To express D2GFP from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native SV40 3'UTR containing two introns

Depositor

Jonathan Ploski

Insert

D2GFP (Arc Synthetic, Rat)

Use

AAV

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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plvx-tetO-GFP

Plasmid

#191560

Purpose

Inducible expression of GFP

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

gfp

Use

Lentiviral

Mutation

None

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP217-Lenti-CMV-GFP-WPRE-pA

Plasmid

#62925

Purpose

pLenti vector backbone designed to express GFP from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.3αCaMKII and CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA

Plasmid

#164787

Purpose

pLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

GFP

Promoter

1.3αCaMKII

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCN34e-ktrA-yfp

Plasmid

#227640

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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