166,289 results

1. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-pHlorina

   Plasmid

   #182763

   Purpose

   pH-sensitive RFP pHlorina for detection of efferocytosis

   Depositor

   Robert Campbell

   Article

   Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

   Insert

   pHlorina
   

   Expression

   Bacterial

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HP17_KO7

   Plasmid

   #120346

   Purpose

   Helper plasmid for production of phagemid ssDNA, based on M13KO7

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Coding part of the M13KO7 helper phage genome
   

   Expression

   Bacterial

   Promoter

   native phage promoters

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-CMV mCherry-GFP-LC3B G120A

   Plasmid

   #123235

   Purpose

   Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag-IDH1-R132H

   Plasmid

   #62907

   Purpose

   Expresses R132 mutant IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   IDH1 (IDH1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted Arg132 (R132)

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. IRES-mCherry

   Plasmid

   #80139

   Purpose

   empty retroviral vector with IRES-mCherry

   Depositor

   Ellen Rothenberg

   Article

   Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Expression

   Mammalian

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-hrGFP II-TET1

   Plasmid

   #83568

   Purpose

   Expresses TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule-3-nitroTyrosine (5B)

   Plasmid

   #85498

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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