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We narrowed to 12,028 results for: LEA

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Showing: 7181 - 7200 of 12028 results

  2. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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  4. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid
    #223136
    Purpose
    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized PEmax-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    M-MLV mutations from PE2 (Anzalone et al. Nature …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223136-plasmid-map-sequence-id-447644

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  5. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid
    #223123
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223123-plasmid-map-sequence-id-447704

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  7. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Synthetic, Photinus pyralis)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  8. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

    Plasmid
    #217799
    Purpose
    Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217799-plasmid-map-sequence-id-432866

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  12. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid
    #210513
    Purpose
    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Inserts
    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV and TetO
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210513-plasmid-map-sequence-id-415575

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  13. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid
    #208955
    Purpose
    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208955-plasmid-map-sequence-id-415744

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  14. pCAGGS-SMS1-TEV-HAT

    Plasmid
    #202557
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Tags
    Histidine-Affinity-tag (HAT) and TEV cleavable si…
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    202557-plasmid-map-sequence-id-399621

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  16. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid
    #140451
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140451-plasmid-map-sequence-id-291874

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  17. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid
    #185485
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185485-plasmid-map-sequence-id-362670

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  18. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid
    #185488
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185488-plasmid-map-sequence-id-362671

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  19. MBP-g0s2

    Plasmid
    #179294
    Purpose
    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    mature maltose binding protein + enterokinase cle…
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    Ptac
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179294-plasmid-map-sequence-id-354917

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Showing: 7181 - 7200 of 12028 results