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pST_neg_DHR93 (pBS0779)

Plasmid

#185252

Purpose

For the mammalian expression of the synthetic protein neg_DHR93. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

neg_DHR93

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHR93_NCcapped (pBS0777)

Plasmid

#185251

Purpose

For the mammalian expression of the synthetic protein DHR93_NCcapped. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHR93_NCcapped

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_TJ190_negative (pBS0773)

Plasmid

#185250

Purpose

For the mammalian expression of the synthetic protein TJ190_negative. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

TJ190_negative

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_SAHS4_k1_0 (pBS0769)

Plasmid

#185249

Purpose

For the mammalian expression of the tardigrade protein SAHS4_k1_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SAHS4_k1_0

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k5_15 (pBS0767)

Plasmid

#185248

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_15. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_15

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k5_1 (pBS0766)

Plasmid

#185247

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_14 (pBS0764)

Plasmid

#185246

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_14. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_14

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_1 (pBS0763)

Plasmid

#185245

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_11 (pBS0761)

Plasmid

#185243

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_11

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k5_0 (pBS0758)

Plasmid

#185242

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k5_0

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_7 (pBS0755)

Plasmid

#185241

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_7

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_DrHD_dom3_k3_1 (pBS0754)

Plasmid

#185240

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CI016_HUMAN (pBS0747)

Plasmid

#185238

Purpose

For the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CI016_HUMAN

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdAPOA4-44 (pBS0745)

Plasmid

#185236

Purpose

For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdAPOA4-44

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8319 pDONR ATG-YAP1 WT-Stop

Plasmid

#184524

Purpose

Subcloning YAP1 WT

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 WT (YAP1 Human)

Use

Unspecified

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6A_L

Plasmid

#146843

Purpose

Insect Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A (TNRC6A Human)

Expression

Insect

Mutation

A339T, K1578E compared to the sequence of isoform…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

Plasmid

#182381

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD2

Plasmid

#180602

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD1

Plasmid

#180601

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRBP-L2+D2+L3-myc/His

Plasmid

#180600

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

Plasmid

#178871

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:S495A

Plasmid

#98151

Purpose

Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:S495A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed serine 495 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:W603A

Plasmid

#98153

Purpose

Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:W603A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tryptophan 603 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:H695Q

Plasmid

#98154

Purpose

Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695Q

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to glutamine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:H695A

Plasmid

#98155

Purpose

Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:Y696G

Plasmid

#98156

Purpose

Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:Y696G

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tyrosine 696 to glycine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D

Plasmid

#146121

Purpose

Insect Expression of DmEDC3_1-101

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-101 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D

Plasmid

#146122

Purpose

Insect Expression of DmEDC3_1-338

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-338 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Tmem209

Plasmid

#175154

Purpose

Lentiviral expression of V5-tagged mouse Tmem209

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem209 (Tmem209 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mSpata33 (I81A)-PA

Plasmid

#179131

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Mutation

Changed Isoleucine 81 to Alanine

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge