We narrowed to 7,672 results for: CCH

1. pKI-cjACTB-EGFP

   Plasmid

   #129747

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

   Plasmid

   #117663

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pOst1-pro-af(MUT2)-E2-Crimson
   

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFL His-I3 V41A / W43A

   Plasmid

   #113515

   Purpose

   Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Tags

   6*His

   Expression

   Insect

   Mutation

   V41A /W43A

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJC20cdc8.27

   Plasmid

   #99362

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Glutamic acid 129 to Lysine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-CAGGS-dCas9-KRAB-MeCP2

   Plasmid

   #110824

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

   Plasmid

   #197044

   Purpose

   In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

   Depositor

   Liqun Luo

   Article

   Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

   Inserts

   pCAG-FSF-LSL
   SP-HA-HRPtm
   WPRE-bGHpolyA
   

   Tags

   HA, myc

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PJ-DEAD

   Plasmid

   #38002

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   FKBP1A (FKBP1A Human)
   SAC1 (SAC1 Budding Yeast)
   INPP5E (INPP5E Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Asp 1263 mutated to Ala and Cys 779 mutated to Ser

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

   Plasmid

   #117661

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-alphaf(I)-E2-Crimson
   

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZ-pre-Ost1-alphaf(I)-Btl2

    Plasmid

    #117667

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-Btl2
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC-G418-YR

    Plasmid

    #61767

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    trpC promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

    Plasmid

    #171619

    Purpose

    AAV floxed Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet-1 TIM9,10

    Plasmid

    #170280

    Purpose

    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

    Insert

    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. yeast_DualReporter

    Plasmid

    #127546

    Purpose

    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

    Depositor

    Aviv Regev

    Article

    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

    Inserts

    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PJ-INPP5E

    Plasmid

    #38001

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1 (SAC1 Budding Yeast)
    INPP5E (INPP5E Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Cys 779 mutated to Ser

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. PJ-Sac

    Plasmid

    #38000

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1 (SAC1 Budding Yeast)
    INPP5E (INPP5E Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Asp 1263 mutated to Ala

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas-pML-1B_6xHis-Cas12m

    Plasmid

    #192282

    Purpose

    MmCas12m fused to His-tag for protein purification

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12m
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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