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We narrowed to 13,842 results for: Cre

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Showing: 7201 - 7220 of 13842 results

  1. pCMV_EE-NG-nCas9

    Plasmid
    #163542
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EE-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163542-plasmid-map-sequence-id-320177

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  2. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid
    #121841
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121841-plasmid-map-sequence-id-237395

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  5. Sema3f(L,+)-AP-His

    Plasmid
    #72021
    Purpose
    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema3f (Sema3f Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72021-plasmid-map-sequence-id-131207

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  8. CyclinB-GFP

    Plasmid
    #54465
    Purpose
    CyclinB-GFP in pIVT vector for in vitro transcription
    Depositor
    Michael Lampson
    Publication
    Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CyclinB
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    K67E and I150T mutations compared to GenBank refe…
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Publication
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. EDNRB-DuET

    Plasmid
    #213233
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EDNRB (EDNRB Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213233-plasmid-map-sequence-id-430896

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  15. CX3C1-DuET

    Plasmid
    #213217
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CX3C1 (CX3CR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213217-plasmid-map-sequence-id-430779

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  19. BE3-NLS-NLS-P2A-BLAST

    Plasmid
    #135348
    Purpose
    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135348-plasmid-map-sequence-id-267697

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  20. H2B-mAvicFP1

    Plasmid
    #129511
    Purpose
    Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)
    Depositor
    Nathan Shaner
    Publication
    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mAvicFP1
    Expression
    Mammalian
    Mutation
    mAvicFP1 fused to the C terminus of human histone…
    Promoter
    CMV
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    129511-plasmid-map-sequence-id-271863

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Showing: 7201 - 7220 of 13842 results