We narrowed to 18,529 results for: IRE

1. 7h sgRNA for 4-μHOM reporter

   Plasmid

   #113625

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7h sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pUE1-TSP-BFLF1

   Plasmid

   #162657

   Purpose

   Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   BFLF1
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual-eGFP

   Plasmid

   #63215

   Purpose

   Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

5. mApple-TfR-RUSH

   Plasmid

   #166906

   Purpose

   RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   mApple
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgTrack-mCherry

   Plasmid

   #114013

   Purpose

   Empty sgRNA expression vector with co-expression of mCherry reporter

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 7i sgRNA for 4-μHOM reporter

   Plasmid

   #113626

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7i sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-Cerulean-Epac2-Venus

   Plasmid

   #138196

   Purpose

   FRET-based biosensor for monitoring Epac2 activation.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. myc-mTOR (1-1482 aa)

   Plasmid

   #21745

   Depositor

   David Sabatini

   Article

   Kim et al Mol Cell 2003 Apr;11(4):895-904.

   Insert

   mTOR (1-1482) (Mtor Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   amino acids 1-1482

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

10. GST-p35

    Plasmid

    #24895

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    p35 (CDK5R1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Full-length p35

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

11. OSCA1.2

    Plasmid

    #136593

    Purpose

    Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgTrack-GFP

    Plasmid

    #114012

    Purpose

    Empty sgRNA expression vector with co-expression of TurboGFP reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPR_Puro_sgAAVS1

    Plasmid

    #187818

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nanog5P reporter

    Plasmid

    #16337

    Depositor

    Austin Cooney

    Article

    Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

    Insert

    Nanog promoter (Nanog Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Nanog promoter

    Available since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

    Plasmid

    #185678

    Purpose

    Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HIR K1030R

    Plasmid

    #24050

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K1030R

    Promoter

    Available since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ H2B-mCherry

    Plasmid

    #99265

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Article

    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

    Insert

    mCherry
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

    Plasmid

    #182560

    Purpose

    Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a ICL1
    sfGFP-P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202023

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-I48V

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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