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pCMV-ABEmax(TadA E59A, TadA*E59A)

Plasmid

#125649

Purpose

pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK Mma PylT (UUA)

Plasmid

#154783

Purpose

plasmid with single copy of Mma PylT (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

U25C and UUA anticodon

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3a_001

Plasmid

#123754

Purpose

Encodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTK

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LYN

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPD107 VP16-ZF43(RARR)

Plasmid

#138812

Purpose

Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RARR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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ROZA

Plasmid

#64198

Purpose

"Reporter of Zap-70 Activity" Fluorescent reporter of ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
MTK4a_014

Plasmid

#123827

Purpose

Encodes a nuclear localized mAzamiGreen coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

P2A::NLS::mAzamiGreen

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_018

Plasmid

#123933

Purpose

Encodes the spacer TU with connectors LS R1 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TULSR1 - spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_019

Plasmid

#123934

Purpose

Encodes the spacer TU with connectors L1 R2 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL1R2 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_020

Plasmid

#123935

Purpose

Encodes the spacer TU with connectors L2 R3 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL2R3 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_022

Plasmid

#123937

Purpose

Encodes the spacer TU with connectors L4 R5 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL4R5 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_023

Plasmid

#123938

Purpose

Encodes the spacer TU with connectors L5 R6 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL5R6 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_024

Plasmid

#123939

Purpose

Encodes the spacer TU with connectors L6 R7 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL6R7 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_025

Plasmid

#123940

Purpose

Encodes the spacer TU with connectors L7 R8 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL7R8 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_026

Plasmid

#123941

Purpose

Encodes the spacer TU with connectors L8 RE as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL8RE spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_025

Plasmid

#123687

Purpose

Encodes ConL3 and human D1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-D1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_026

Plasmid

#123688

Purpose

Encodes ConL4 and human E1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-E1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPD1122 CMV 3xFLAG-NES-PYL1-VP64 in TUPV3

Plasmid

#161547

Purpose

Constitutive expression of 3xFLAG-NES-PYL1-VP64 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NES-PYL1-VP64

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MTK8a_005

Plasmid

#123889

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK7_012

Plasmid

#123879

Purpose

Encodes the 5' 500 bp hCYBYL locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hCLYBL

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_002

Plasmid

#123891

Purpose

Encodes the 3' 500 bp hRosa26 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hROSA26

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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