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pAAV-CaMKII-pAceR-Kv2.1PR

Plasmid

#195531

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1

Plasmid

#61086

Purpose

Express N-terminal GST tagged-mouse BIN1 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

smallest constitutive BIN1excluding exon 7, 11, 13-17

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pRSETa-mEos3.2-A69T

Plasmid

#98571

Purpose

photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos3.2-A69T

Tags

7xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTBL437 AAVS1 EQR sgRNA

Plasmid

#126447

Purpose

To cut the human AAVS1 locus for integration.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human AAVS1 safe-harbor locus with NGAG PAM

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Tags

mRuby3

Expression

Yeast

Promoter

pHis3

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg1

Plasmid

#139450

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Axin1_HA in pRK5

Plasmid

#154220

Purpose

C-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

AXIN1 (AXIN1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-ITFG2

Plasmid

#87049

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-mHopX

Plasmid

#16425

Depositor

Jonathan Epstein

Article

Chen et al Cell. 2002 Sep 20. 110(6):713-23.

Insert

Hopx (hop Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV tubulin myc

Plasmid

#12581

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

tubulin (TUBG1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

*

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pKS18

Plasmid

#24629

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

mFar1 (Far1 Mouse)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only

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JPF0419d

Plasmid

#124020

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLX302-MKP7-V5 puro

Plasmid

#87771

Purpose

Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP7 (DUSP16 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-SETDB1_1

Plasmid

#36347

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SETDB1 (SETDB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-SFXN5

Plasmid

#110639

Purpose

Retroviral expression of Flag-SFXN5 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN5 (SFXN5 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-MYC-ITFG2

Plasmid

#87046

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- WTN23C11orf83-GFP

Plasmid

#65845

Purpose

Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

WT Transmembrane

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-hDcp2 E147Q, E148Q

Plasmid

#72215

Purpose

bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

His

Expression

Bacterial

Mutation

E147Q, E148Q

Promoter

T7

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-Bmi1_2

Plasmid

#36353

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
Lenti-SLC35B2-sgRNA

Plasmid

#154860

Purpose

Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 sgRNA (SLC35B2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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