We narrowed to 16,181 results for: nes
-
Plasmid#100710PurposeB52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro HA-Atg14*dCC
Plasmid#38266DepositorInsertautophagy related genes 14 (ATG14 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged A618T, A621T, C624A and T627C for siRNA r…PromoterLTRAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pvPE-V4-Puro
Plasmid#240290PurposeEncodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpvPE-V4-Puro
UseCRISPRTagsSV40 NLS and c-Myc NLSExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1
Plasmid#205443Purposehuman AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCD46-HLA-G SCT-hCD47-hCD59
ExpressionMammalianPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IMutationmutant KRAS4A-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
B52 + CHEK2 sgSTOP
Plasmid#100712PurposeB52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dGZmxh
Plasmid#44552DepositorInsertsPTETREG promoter
yEGFP::ZeoR
UseSynthetic Biology; Expression regulator/reporterExpressionYeastPromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-P459L
Plasmid#25644DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseLentiviralExpressionMammalianMutationtumor-derived mutation created by site-directed m…Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PRKRA
Plasmid#20608DepositorPublicationAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
Plasmid#173862PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCAGAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)
Plasmid#49063PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRevTRE MKL1-N100 S449A/T450A/S454A
Plasmid#19849DepositorInsertMKL1-N100 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGMutationdeleted amino acids 1-100 and changed Serine 449,…Available sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNOC-RLUC
Plasmid#98142PurposeGateway compatible construct for N' terminal fusion or promoter driven renilla luciferase reporter, hygromycin resistance cassetteDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsHAAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSW24
Plasmid#86837Purposebacterial expression of anti-CRISPR protein AcrIIA3DepositorInsertacrIIA3
ExpressionBacterialPromoteraraBADAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSW65
Plasmid#86838Purposebacterial expression of anti-CRISPR protein AcrIIA3DepositorInsertacrIIA3
ExpressionBacterialPromoteraraBADAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179436PurposeDual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHS4-TRE-cit-HS4-mch (DH)
ExpressionMammalianPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V
Plasmid#159542PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12VDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12VAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TC9nickpAB
Plasmid#71311Purposeused as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsIDepositorInsertCas9 nickase
UseIn vitro transcriptionTagsNLSMutationD10A nickasePromoterT7Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBDU
Plasmid#62661PurposeTargeting vector for PITT landing pad knock-in into the Rosa26 locusDepositorInsertseGFP
neomycin
UseMouse TargetingAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only