We narrowed to 22,175 results for: TES

1. pETM33_SPSB1_SPRY

   Plasmid

   #178487

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SPSB1_SPRY (SPSB1 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D

   Plasmid

   #146123

   Purpose

   Insect Expression of DmEDC3_1-440

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   DmEDC3_1-440 (Edc3 Fly)
   

   Expression

   Insect

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG.INBox-mCherry-INBox-eDHFR

   Plasmid

   #176203

   Purpose

   Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   INBox-mCherry-INBox-eDHFR
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pStr-KDEL_APV-SBP-Dendra2-ER-5

   Plasmid

   #170679

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   APV-SBP-Dendra2-ER-5
   streptavidin-KDEL
   

   Expression

   Mammalian

   Promoter

   IRES and T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA

   Plasmid

   #178869

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HA

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-mCherry-Zdk1-Vav2cat

   Plasmid

   #178875

   Purpose

   Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG.mCherry-INBox-eDHFR

   Plasmid

   #176202

   Purpose

   Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   mCherry-INBox-eDHFR
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG.mCherry-INBox(AAA)-eDHFR

   Plasmid

   #176207

   Purpose

   INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   mCherry-INBoxAAA-eDHFR
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+-wbp2nlY282F

   Plasmid

   #172468

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlY282F
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   changed Tyrosine at aa282 to Phenylalanine

   Promoter

   SP6

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174528

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Insert

    PylS
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ecm2 1-197

    Plasmid

    #169924

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-197
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_hNKD2

    Plasmid

    #174329

    Purpose

    encodes human NKD2 cDNA with a C-terminal MycHis Tag.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Tags

    MycHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH775_pJH_LldR_PlldR-sfGFP.2opal

    Plasmid

    #163734

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding two opal codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.2TGA
    

    Expression

    Bacterial

    Mutation

    gfp with first two leucine codons mutated to amber

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+-wbp2nlF127P

    Plasmid

    #174507

    Purpose

    expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlF127P
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Phenylalanine at aa127 to Proline

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+-wbp2nlT45A

    Plasmid

    #172464

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlT45A
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Threonine at aa45 to Alanine

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+-wbp2nlY55F

    Plasmid

    #172466

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY55F
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Tyrosine at aa55 to Phenylalanine

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AraC-pHG165c WT

    Plasmid

    #170114

    Purpose

    Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    AraC
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

    Plasmid

    #170792

    Purpose

    This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPR V2 sgACO1_3

    Plasmid

    #169893

    Purpose

    Disrupt ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting ACO1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTX1-SMN2-exon7-ESE1

    Plasmid

    #126042

    Purpose

    In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN2 exon7 ESE1
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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