We narrowed to 17,800 results for: Jun
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pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2mut
Plasmid#203606PurposeReporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequencesDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseExpressionMammalianMutationmiR-27b target sequences in the 3'UTR were m…PromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in mammalian cellsDepositorInsertCodon-optimized wild type Bxb1
ExpressionMammalianPromoterCMVAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-Pol2-ffLuc2-eGFP
Plasmid#71394PurposeLentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoterDepositorInsertPol2-ffLuc2-eGFP
UseLentiviralExpressionMammalianPromoterPol2 (mouse RNA polymerase II gene promtoer)Available SinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
OMMGFP 1-10
Plasmid#164119PurposeExpresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMMDepositorInsertOMMGFP 1-10
ExpressionMammalianAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 WT
Plasmid#12605DepositorAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
EF-hGPC3
Plasmid#24670DepositorAvailable SinceMay 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralExpressionBacterial and MammalianAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
LADL Anchor
Plasmid#127664PurposeEncodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoterDepositorInsertdCas9-CIBN-2A-Puro
Tags3XFLAGExpressionMammalianMutationCas9 (D10A,H840A) mutantPromoterEF1aAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-ECFP
Plasmid#112220PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFPDepositorInsertECFP
UseCRISPRExpressionMammalianAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MCS-GFP-SV-puro
Plasmid#73582PurposeThird generation lentiviral vector expressing GFP and puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsGFP tagAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10ExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPPromoterEF1aAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EPS15-GFP-His
Plasmid#170860PurposeMammalian expression of EPS15-GFP-His.DepositorInsertEpidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)
Tags(His)6 and EGFPExpressionMammalianPromoterCMVAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-5p
Plasmid#103357PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-218-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2
Plasmid#164510PurposeGreen glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.DepositorHas ServiceAAV9Insert(Cyto)iGlucoSnFR-mRuby2
UseAAVMutationSynthetic ConstructPromoterSynapsinAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GPHN.FingR-mKate2-IL2RGTC
Plasmid#46297DepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only
