We narrowed to 17,934 results for: multi
-
Plasmid#223213PurposeLentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)
Plasmid#164193PurposeFreeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationF391W-L398VPromoterSynAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Pard3-EGFP-PIF6
Plasmid#154914PurposeFull length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).DepositorInsertpar-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
UseXenopus/avian/zebrafishTags1-100 amino acids of Arabidopsis PIF6 and EGFPExpressionMammalianPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-LRP4 (cytoplasmic) [N164/6.1R]
Plasmid#114542PurposeMammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.DepositorInsertanti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
ExpressionMammalianPromoterdual CMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorExpressionBacterialPromotercat promoter and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4495
Plasmid#80338Purposemammalian expression MbCpf1 nuclease and Mb crRNADepositorInsertsMb crRNA
MbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGD_MiniTurboID-YFP
Plasmid#222434PurposeGolden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGB_YFP-MiniTurboID
Plasmid#222433PurposeGolden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMT-MjtRNA-GFP(47TAG)
Plasmid#193251PurposeExpresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)DepositorInsertsGFP(47TAG)
MjtRNA
ExpressionBacterialMutationCodon for Tyr47 replaced with amber stop codon TA…Promoterhsp60 and metUAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Gluc-RMA-IRES-EGFP
Plasmid#189629PurposeExpresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianPromoterIRES and hSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIV537
Plasmid#177704PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
Plasmid#178528PurposeExpresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertrtTA, SspB-mScarlet-I-MYPT (1-169aa)
ExpressionMammalianPromoterCAG, Tet responsive elementAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir451-CMV2-mir1
Plasmid#164635PurposeExpress miR-451 and miR-1-1 in mammalian cellsDepositorAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA
Plasmid#113692PurposeU6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsNLSPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLV-gag-hMLH1dn
Plasmid#195513PurposeMMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.DepositorInserthMLH1dn (MLH1 Human)
UseVirus like particleTagsFLAG, NES, and Protease cleavate siteExpressionMammalianMutationHuman MLH1 del754-756PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 G2032R
Plasmid#183814PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cellsDepositorUseRetroviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationG2032RPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-Sec-APEX2m
Plasmid#176845PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-Sec-APEX2m
ExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP6
Plasmid#128940PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, ZDepositorInsertsTagsHisExpressionBacterialMutationPreScission protease site (GE Healthcare; LEVLFQG…PromoterT7Available sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Anti-Mortalin/GRP75 [N52A/42.1R]
Plasmid#114548PurposeMammalian Expression Plasmid of anti-Mortalin/GRP75 (Mouse). Derived from hybridoma N52A/42.1.DepositorInsertanti-Mortalin/GRP75 (Mus musculus) recombinant mouse monoclonal antibody (Hspa9 Mouse)
ExpressionMammalianPromoterdual CMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC-repo1.9kb-TALE4-VP64-P10
Plasmid#104612PurposeExpresses TALE4 under the control of a 1.9kb repo enhancer elementDepositorAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only