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pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

Plasmid

#153956

Purpose

Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF7B C-trunc

Plasmid

#153957

Purpose

Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC45A3

Plasmid

#132167

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC45A3 (SLC45A3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLX403_SMARCB1_W281R

Plasmid

#250673

Purpose

Inducible vector with SMARCB1 variant 1

Depositor

Andrew Hong

Article

Cooper et al Nat Commun. 2026 Apr 8. doi: 10.1038/s41

Insert

SMARCB1 (SMARCB1 Human)

Expression

Mammalian

Mutation

Tryptophan 281 to Arginine

Promoter

TRE

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pME-FastFucci-P2A-H2A-tdiRFP (JDW 1399)

Plasmid

#242557

Purpose

Gateway middle entry clone containing mammalian FastFUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state. tdiRFP reporter labels all cells regardless of cell cycle state

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Use

Gateway Cloning

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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p5E-hs_UbiC-pro (JDW 1461)

Plasmid

#242547

Purpose

Enhancer/Promoter: Gateway 5' entry vector containing human Ubc promoter (-1225 to -6)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Ubiquitin / Ubc promoter (UBC Human)

Use

Gateway Cloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-ITGB1(no stop)

Plasmid

#215453

Purpose

Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

Sept. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME-EGFP-Myc (JDW 1441)

Plasmid

#242569

Purpose

Gateway middle entry clone containing EGFP fused to oncogenic c-Myc.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

c-Myc (MYC Human)

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(YYFF)

Plasmid

#215451

Purpose

Gateway entry vector with integrin beta1 NPxY mutant (YYFF)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-Crestin-pro (JDW 1320)

Plasmid

#229830

Purpose

A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Crestin promoter, c-fos minimal promoter, and b-globin intron

Use

Gateway Cloning

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC-GFP-IRES-mCherry

Plasmid

#231232

Purpose

MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MCRS1-GFP-IRES-mCherry

Plasmid

#231231

Purpose

MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-F97W

Plasmid

#203572

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Phenylalanine 97 to Tryptophan for partia…

Promoter

CMV

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209061

Purpose

Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

KDM6A

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(T)

Plasmid

#206143

Purpose

Expresses the rs27895-T allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Tags

myc

Mutation

G346 changed to D346

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC2A13_STOP

Plasmid

#161316

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A13 (SLC2A13 Human)

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC35F1_STOP

Plasmid

#161299

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F1 (SLC35F1 Human)

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC35E3_STOP

Plasmid

#161228

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E3 (SLC35E3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC2A12_STOP

Plasmid

#161233

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A12 (SLC2A12 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC35D3_STOP

Plasmid

#161276

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D3 (SLC35D3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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