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Showing: 7261 - 7280 of 31746 results

  3. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217345
    Purpose
    crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81
    Depositor
    Luke Gilbert
    Publication
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
    crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
    crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
    crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    217345-plasmid-map-sequence-id-426760

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  4. pCDNA5-LRRK2

    Plasmid
    #229019
    Purpose
    Expression of untagged full length human LRRK2 in mammalian cells
    Depositor
    Samara Reck-Peterson
    Publication
    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Leucine-rich repeat kinase 2 (LRRK2 Human)
    Tags
    no tags (untagged)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    229019-plasmid-map-sequence-id-454988

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  6. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

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  8. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid
    #188524
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2XMTS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188524-plasmid-map-sequence-id-372835

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  10. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid
    #176458
    Purpose
    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176458-plasmid-map-sequence-id-346303

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  11. Equalizer-L episome

    Plasmid
    #169738
    Purpose
    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169738-plasmid-map-sequence-id-334158

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  12. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

    Plasmid
    #169921
    Purpose
    DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IRES-Puro-pEF1a-EGFP
    Use
    CRISPR
    Promoter
    EF1a
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169921-plasmid-map-sequence-id-336026

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  13. Equalizer-L plasmid

    Plasmid
    #169732
    Purpose
    Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169732-plasmid-map-sequence-id-334139

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  14. pCMV-MtAcKRS (human-opti)

    Plasmid
    #164195
    Purpose
    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
    Depositor
    Tao Liu
    Publication
    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MtAcKRS-478(human-opti)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    D76G; L325M; L329I; Y330F; L333A; C372F
    Promoter
    CMV
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    164195-plasmid-map-sequence-id-325763

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  17. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid
    #133789
    Purpose
    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CjeCas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133789-plasmid-map-sequence-id-268569

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Showing: 7261 - 7280 of 31746 results