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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-F152E-V5His6_AC

Plasmid

#148467

Purpose

Insect Expression of DmNanos_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-F152E (nos Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-L127DF130D-V5His6_AC

Plasmid

#148468

Purpose

Insect Expression of DmNanos_116-163-L127DF130D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-L127DF130D (nos Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1710-2505-H2067D-dsRNAres_AD

Plasmid

#148500

Purpose

Insect Expression of DmNot1_1710-2505-H2067D-dsRNAres

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNot1_1710-2505-H2067D-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_50-236-del116-163-GB1opt_AB

Plasmid

#148330

Purpose

Bacterial Expression of DmNanos-opt_50-236-del116-163

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_50-236-del116-163 (nos Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot3_607-748-Y702A-NpM-HsNot2_350-540_AB

Plasmid

#148334

Purpose

Bacterial Expression of HsNot3_607-748-Y702A-HsNot2_350-540

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

HsNot3_607-748-Y702A-HsNot2_350-540 (CNOT3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-hfCas13d-pA

Plasmid

#195866

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1312-1355-DmDCP1_1-127_N

Plasmid

#147054

Purpose

Bacterial Expression of DmXRN1_1312-1355-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1312-1355-DmDCP1_1-127 (pcm Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1355-DmDCP1_1-127_N

Plasmid

#147055

Purpose

Bacterial Expression of DmXRN1_1323-1355-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355-DmDCP1_1-127 (pcm Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N

Plasmid

#147056

Purpose

Bacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1383-DmDCP1_1-127 (pcm Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P

Plasmid

#147220

Purpose

Bacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 (pcm Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P

Plasmid

#147209

Purpose

Insect Expression of DmXRN1-P1339AP1342A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-P1339AP1342A-dsRNAres (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

Plasmid

#148466

Purpose

Insect Expression of DmNanos_116-163-E151AF152AN1555A

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-E151AF152AN1555A (nos Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-R349A-EFGP

Plasmid

#176898

Purpose

mouse ILK with R349A point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 349 to Alanine

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-R255A-EFGP

Plasmid

#176897

Purpose

mouse ILK with R255A point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 255 to Alanine

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLB(N)CX-FLAG-CH3 Del33

Plasmid

#133724

Purpose

Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag

Mutation

partial (not complete) p300 fragment, AT and CH3 …

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGDF11 #2

Plasmid

#83084

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA5-puro-eGFP-HEC1-M4

Plasmid

#114036

Purpose

Expression of HEC1 M4 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M4 (NDC80 Human)

Expression

Mammalian

Mutation

M4 (K146E, R153E, K156E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-M2

Plasmid

#114035

Purpose

Expression of HEC1 M2 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M2 (NDC80 Human)

Expression

Mammalian

Mutation

M2 (K81E, N87E, K89E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4 WW2 neg ctrl

Plasmid

#112030

Purpose

Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Insert

NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry

Tags

Split mCherry

Expression

Bacterial

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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