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KSR1-HaloTag Fusion Vector

Plasmid

#236794

Purpose

Express KSR1-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

KSR1 (KSR1 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

Plasmid

#246430

Purpose

All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

O.gue IscB with dead mutations (D60A, H269A) and delta-TID
mCherry
ωRNA

Tags

SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

Expression

Mammalian

Mutation

changed Aspartic Acid 60 to Alanine, changed Hist…

Promoter

CMV and U6

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#219782

Purpose

CaST-IRES for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

Plasmid

#165445

Purpose

Expresses ACX, rPB(8kD) in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rPB(8kD)

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-mCherry2-G4

Plasmid

#164167

Purpose

Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

Plasmid

#232879

Purpose

Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

5-Hydroxytryptamine Receptor 6 (HTR6 Human)

Use

Lentiviral

Tags

RGECO1.1

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

Plasmid

#228729

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTb(C)-M13-GFP

Plasmid

#219780

Purpose

sTb(C) half CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-CMV-TFAM-BioID2-IRES-Puro

Plasmid

#211465

Purpose

Lentivirus backbone expressing human TFAM fused with BioID2

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

TFAM-BioID2

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-CAG-GFP-EnvA(N2c)

Plasmid

#194353

Purpose

Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain

Depositor

Marco Tripodi

Article

Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

Inserts

Enhanced Green Fluorescent Protein (eGFP)
EnvA-CVS-N2c Rabies Glycoprotein

Use

Lentiviral

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) RALB

Plasmid

#107506

Purpose

Expressing RALB, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

RALB (RALB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) CRIP2

Plasmid

#107509

Purpose

Expressing CRIP2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

CRIP2 (codon optimized) (CRIP2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p252 pPr-CREM/CMV-STOP-luc

Plasmid

#8406

Purpose

Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin promoter, CMV

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pLV-Puro-{CMV-CuO}>{PAX3::FOXO1}:T2A:mCherry

Plasmid

#236629

Purpose

Puromycin selected lentiviral construct with cumate-inducible PAX3::FOXO1

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

PAX3::FOXO1 (FOXO1, PAX3 Human)
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-CuO

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

Plasmid

#214732

Purpose

Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA / mCherry

Use

AAV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

Plasmid

#210725

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

C2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugABE-NNG-P2A-puro

Plasmid

#214354

Purpose

Expresses SlugABE-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-NNG-P2A-puro

Plasmid

#214352

Purpose

Expresses SlugCas9-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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