We narrowed to 77,526 results for: Mycs;

1. attB-mCh-Dnmt3b1-Poly(A)-PuroR

   Plasmid

   #65556

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 1

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lyn-FLINC-EKAR1

   Plasmid

   #87707

   Purpose

   Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   Lyn-FLINC-EKAR1
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. attB-mCh-Dnmt3b6-Poly(A)-NeoR

   Plasmid

   #65554

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 6

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lyn-FLINC-EKAR1(TA)

   Plasmid

   #87708

   Purpose

   Negative-control mutant of Lyn-FLINC-EKAR1.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   Lyn-FLINC-EKAR1(TA)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZE21/UBP1/ClpS

   Plasmid

   #98566

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   In a synthetic operon downstream of UBP1 and Remo…

   Promoter

   Same as UBP1 (operon) and Tet promoter (aTc induc…

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBGE1.0

   Plasmid

   #165986

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA1B1C1
   nourseothricin N-acetyl transferase
   spectinomycin/streptomycin resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. His6-SMT3-SopB(C460S)

   Plasmid

   #183677

   Purpose

   Recombinant Salmonella Typhimurium SopB (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   C460S

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. G30, Cdc20-mCherry-AID tagging

   Plasmid

   #130270

   Purpose

   Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

   Depositor

   Matthias Heinemann

   Article

   Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

   Insert

   Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-mCh-Dnmt3b1-Poly(A)-NeoR

   Plasmid

   #65553

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 1

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. attB-GFP-Dnmt3b1-Poly(A)-NeoR

    Plasmid

    #65551

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 1

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SSH-pSUMO-β′-COPI-WD40-Lys17Ala

    Plasmid

    #208171

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
    

    Use

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Mutation

    Lys17Ala substitution

    Promoter

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS

    Plasmid

    #221117

    Purpose

    FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    MFA-IgKappa-FAPoptim-STE3-Tailless
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    STE3 mutant that lacks the entire C-terminal tail…

    Promoter

    STE3

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM88

    Plasmid

    #226680

    Purpose

    Rpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6)

    Depositor

    Andreas Martin

    Article

    Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

    Inserts

    rpt1 (RPT1 Budding Yeast)
    Rpt2 (RPT2 Budding Yeast)
    Rpt3 (RPT3 Budding Yeast)
    Rpt4 (RPT4 Budding Yeast)
    Rpt5 (RPT5 Budding Yeast)
    Rpt6 (RPT6 Budding Yeast)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    I191TAG

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM89

    Plasmid

    #226681

    Purpose

    Rpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, Rpt6)

    Depositor

    Andreas Martin

    Article

    Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

    Inserts

    rpt1 (RPT1 Budding Yeast)
    Rpt2 (RPT2 Budding Yeast)
    Rpt3 (RPT3 Budding Yeast)
    Rpt4 (RPT4 Budding Yeast)
    Rpt5 (RPT5 Budding Yeast)
    Rpt6 (RPT6 Budding Yeast)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Q49TAG

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid

    #208172

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    

    Use

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

    Plasmid

    #208173

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
    

    Use

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

    Plasmid

    #208174

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    

    Use

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TO-EGFR*-CUbo-GFP

    Plasmid

    #212815

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    8xHis and CUbo-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT-TO-EGFR*-CUbo-His6

    Plasmid

    #212819

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    CUbo-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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