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pUC18T-mini-Tn7T-hph-Para

Plasmid

#218363

Purpose

Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#221134

Purpose

Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

Depositor

Daniel Daley

Publication

Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

Inserts

[PibpA-gfpASV]
[PcpxPmcherryASV] OPT

Expression

Bacterial

Promoter

cpxP and ibpA

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ6332 - ACTB CRISPRa CLIP Donor

Plasmid

#210015

Purpose

CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

Depositor

Stanley Qi

Publication

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

dLbCpf1-miniVPR-T2A-GFP

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Publication

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P**-R

Plasmid

#189851

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Publication

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P**-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P** (modified T7 phage gene 10)

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Publication

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAV0783

Plasmid

#133515

Purpose

Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0756

Plasmid

#133508

Purpose

Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBsaGSCYP101A1

Plasmid

#108206

Purpose

CYP101A1

Depositor

Teruyuki Nagamune

Publication

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

CYP101A1

Expression

Bacterial

Promoter

lac promoter

Available since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-CAF1-C-10

Plasmid

#57351

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CAF1

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCS1

Plasmid

#55749

Purpose

Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Publication

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-Phelo

Plasmid

#48995

Purpose

Gateway adapted transfer of Phleomycin resistance gene

Depositor

Rick Tarleton

Publication

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

Sh ble

Use

Gateway attb1 & attb2

Available since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pfastbacHTb/mClock26-384

Plasmid

#47336

Purpose

mClock fragment cloned into pfastbacHTb vector with Hexa His tag

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

His and Tev

Mutation

aa 26-384

Promoter

PH

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pVRb15_up436

Plasmid

#49709

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf15_up436

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb03_up1198

Plasmid

#49706

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf03_up1198

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pFAB3725

Plasmid

#47837

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 6 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pfastbac/mBmal62-447

Plasmid

#47335

Purpose

mBmal fragment cloned into pfastbac and crystallized

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Mutation

aa 62-447

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
V32V

Plasmid

#29561

Depositor

Steven Vogel

Publication

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-32-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only