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POT3-RFP

Plasmid

#65319

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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POT9-RFP

Plasmid

#65325

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-URR1

Plasmid

#65331

Purpose

standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1

Use

Synthetic Biology

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

Plasmid

#46593

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16 Decoy (Mir16-1 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
mIFP-MyosinIIC-N-18

Plasmid

#56232

Purpose

Localization: Cytoskeleton, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MyosinIIC

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Clover-ZO1-C-14

Plasmid

#56375

Purpose

Localization: Tight Junctions, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

ZO1 (TJP1 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

Plasmid

#69226

Purpose

Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ914

Plasmid

#44489

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]

Use

Gateway expression clone

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only