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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Publication

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Tags

3xFlag, His, and T7

Expression

Mammalian

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBabe EGFR Del5

Plasmid

#32063

Depositor

Matthew Meyerson

Publication

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete E746-S752, insert V)

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del4

Plasmid

#32064

Depositor

Matthew Meyerson

Publication

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete L747-P753, insert S

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pENTR/U6 HDAC5 shRNA

Plasmid

#32222

Depositor

Reuben Shaw

Publication

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against mouse HDAC5 (Hdac5 Mouse)

Use

RNAi; Gateway u6 entry vector

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Publication

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Vh-HA-3xFKBP

Plasmid

#20222

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.2 HA

Plasmid

#12583

Depositor

David Sabatini

Publication

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pOmnibac_ZZ_linker_K963_GFP_SNAP

Plasmid

#159727

Purpose

Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Publication

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

Plasmid

#149714

Purpose

Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

Depositor

Silvia Monticelli

Publication

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K1268R CRres si2,4R

Plasmid

#139405

Purpose

Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Publication

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K1268R

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_S94A-PolyA

Plasmid

#112286

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Serine 94 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

Plasmid

#51255

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Ala

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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DCGS-10

Plasmid

#24752

Depositor

William Pavan

Publication

Southard-Smith et al Nat Genet. 1998 Jan . 18(1):60-4.

Insert

(sex determining region Y)-box 10 (Sox10 Mouse)

Use

In situ probe template

Mutation

3' end of Sox10 cDNA for in situ probe. Tran…

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEBG-TFII-I gamma

Plasmid

#22156

Depositor

Ananda Roy

Publication

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available since

Dec. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Publication

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#223173

Purpose

Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

Depositor

Neville Sanjana

Publication

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1479

Plasmid

#143504

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP63 (TP63 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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His-Tev-Nedd4L

Plasmid

#186806

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

Depositor

Lalit Deshmukh

Publication

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-Nedd4L (NEDD4L Human)

Tags

His6-Tev

Expression

Bacterial

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSOX9-dTAG-mNeonGreen-V5

Plasmid

#194971

Purpose

AAV vector for knocking in a C-terminal tag (dTAG-mNeonGreen-V5) for human SOX9

Depositor

Joanna Wysocka

Publication

Naqvi et al Nat Genet. 2023 May;55(5):841-851. doi:

Insert

SRY-box transcription factor 9 (SOX9 Human)

Use

AAV

Tags

dTAG-mNeonGreen-V5

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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