Purpose
Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
Inserts
Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
Mutation
Leucine 212 changed to proline, leucine 434 in th…
Available Since
Aug. 29, 2024
Availability
Academic Institutions and Nonprofits only