We narrowed to 7,357 results for: Ank
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Plasmid#228562PurposeEncodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.DepositorAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only
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pDONR223-KSR2
Plasmid#23501DepositorInsertKSR2 (KSR2 Human)
UseGateway donor vectorMutationaa344-5 (DP) deleted; S523G compared to GenBank E…Available SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
Plasmid#156415Purposekdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseZebrafish transgenesisTagsmTagBFP2Mutationmutant KRAS4A-G12DPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AMHR2
Plasmid#20424DepositorInsertAMHR2 (AMHR2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationC473G mutation when compared to Genbank reference…Available SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IMutationmutant KRAS4A-G12VPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LRRN2-Fc(DAPA)-AviTag-6xHis
Plasmid#156928PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertLRRN2 (LRRN2 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCerulean
Plasmid#66084PurposeG protein alpha-s internally tagged with mCerulean and EE epitopeDepositorInsertG-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
Tagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPMutationmutant KRAS4B-G12VPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MKC86_HBA1(tEPOR-2A-YFP)
Plasmid#232405PurposeAAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.DepositorUseAAVExpressionMammalianAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
KRD9_HBA1(HBA1reg-HBB)
Plasmid#232402PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank HBA1 cassette. HAs are ~400bp each.DepositorAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
KRD2_HBA1(HBA1reg-UbC-GFP)
Plasmid#232401PurposeAAV production plasmid for HBA1 WGR vector from Fig. 1 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBA1 UTRs flank UbC-GFP cassette; GFP is followed by BGH polyA. HAs are ~400bp each.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]
Plasmid#196887PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ZKSCAN1_Tau10-12K317M
Plasmid#194164PurposeInsert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.DepositorInsertMicrotubule-associated protein tau 10-12 WT (MAPT Human)
Tags3X FlagExpressionMammalianMutationChanged Lysine 317 to MethionineAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
Plasmid#156413Purposekdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sitesDepositorAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ABCB9_WT_V5
Plasmid#82946PurposeGateway Donor vector containing ABCB9, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertABCB9 (ABCB9 Human)
UseGateway entry vectorMutationAA 582- 590 VCARAWATL; AA 592-595 RPFC, AA 597-68…PromoterNoneAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoA
Plasmid#196051PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai3
Plasmid#196050PurposeEncodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only