We narrowed to 10,491 results for: fus
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Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GA-MFF
Plasmid#141160PurposeDimerization-dependent green fluorescent protein targeted to mitochondrial outer membraneDepositorAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK375 (Hygro-P2A-Stag-3FLAG)
Plasmid#121186PurposeN-terminal taggingDepositorInsertHygro-P2A-Stag-3FLAG
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
ExpressionBacterialPromoterPtetAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LacI INCENP
Plasmid#45236PurposeTarget INCENP to lac operator sequenceDepositorInsertLacI, INCENP-Δcen, mCherry (lacI )
TagsLacI (lac repressor protein) and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Promotert7Available SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)
Plasmid#30185DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZP06B
Plasmid#232321PurposeMake sacB a counterselection marker to replace galKDepositorInsertPrpsJ-sacB
ExpressionBacterialAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBigTB-CreERT2
Plasmid#149434Purposetamoxifen-inducible Cre recombinaseDepositorInsertCre-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianPromoterCAG promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS Omomyc-Mer
Plasmid#113170PurposeExpression of Omomer (tamoxifen inducible Omomyc) in mammalian cellsDepositorInsertOmomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
ExpressionMammalianMutationE57T, E64I, R70Q, R71NAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ GEMIN6 myc HIS
Plasmid#71659PurposeMammalian expression of GEMIN6DepositorAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
ExpressionYeastPromoterpFUS1J2Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)
Plasmid#114086PurposeCAG promoter expression plasmid for human codon optimized LbBE1.4DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationD832APromoterCAGAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KozATG-dL5-2XG4S-actin
Plasmid#73262PurposeExpresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertKozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
TagsThe FAP and actin are fused with 2 copies of a G4…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ADARcd-YTHD422N
Plasmid#194402PurposeADAR1 catalytic domain E488Q fused to YTHD422NDepositorInsertADARcd fused to YTHD422N (ADAR Human)
TagsADARcd-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-3HA
Plasmid#17373DepositorInsertWnt-3HA (WNT3 Human, Mouse)
TagsHAExpressionBacterialMutationThis gene is a fusion of the murine and human wnt…Available SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only