We narrowed to 17,014 results for: EMB

1. pFP.R364

   Plasmid

   #138234

   Purpose

   Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFP.R274

   Plasmid

   #138223

   Purpose

   Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL-L1-1.3-KRAB

   Plasmid

   #103034

   Purpose

   [Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-1.3-KRAB
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   HA and KRAB

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-Z21

   Plasmid

   #120064

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   24xSunTag
   

   Use

   Pcr template

   Tags

   24xSunTag

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE:shRNA A-pGK:rtTA-Cre

   Plasmid

   #68471

   Purpose

   Inducible lentivirus expressing an arbitrary shRNA and Cre

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   GFP-shRNA A-miR30
   rtTA-2A-Cre
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   pGK and pTRE

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. OPN(-213)-luc

   Plasmid

   #31107

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

   Insert

   osteopontin promoter (SPP1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   promoter region -213 to +87

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-RhebL1

   Plasmid

   #133766

   Purpose

   Expresses GFP-tagged RhebL1 in mammalian cells

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   RhebL1 (RHEBL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMB-NUCr

   Plasmid

   #61169

   Purpose

   Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   NLS-mCherry-GUS
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   MtBCP1

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDDGFP-Leu2d-ScVrg4

   Plasmid

   #102334

   Purpose

   Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

   Insert

   YGL225W (VRG4 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL2-GGA(EAAAK)2AGG

    Plasmid

    #137104

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    GGA(EAAAK)2AGG
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TOPO pTRE

    Plasmid

    #68457

    Purpose

    TOPO construct expressing pTRE for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Tetracycline Response Element Promoter
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-FLAG-GFP-Ubl4A

    Plasmid

    #86921

    Purpose

    To express Ubl4A tagged with FLAG-EGFP at N- terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    UBL4A (UBL4A Human)
    

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSERT-N1-pECFP

    Plasmid

    #70106

    Purpose

    mammalian expression of hSERT with C-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

    Insert

    SERT (SLC6A4 Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-eGtACR1-mRuby2-ST

    Plasmid

    #109136

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRc/CMV HA TrkB-T1

    Plasmid

    #39980

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkB.T1 (Ntrk2 Rat)
    

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    TrkB.T1 variant; endogenous signal sequence was r…

    Promoter

    CMV

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pF(UG) hSyn spCas9

    Plasmid

    #179725

    Purpose

    human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    spCas9
    

    Use

    Lentiviral

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PIGAZFN-L1

    Plasmid

    #37797

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    PIGA zinc finger L1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. RP2-EGFP

    Plasmid

    #86073

    Purpose

    A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-P09

    Plasmid

    #120020

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mVenus
    

    Use

    Pcr template

    Tags

    mVenus

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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