We narrowed to 3,702 results for: WOL

Showing: 361 - 370 of 3702 results

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pLKO1-puro-U6-sgRNA-TS3

Plasmid

#69235

Purpose

U6 driven SpCas9 sgRNA expression for TS3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS4

Plasmid

#69236

Purpose

U6 driven SpCas9 sgRNA expression for TS4 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pPBT-PE2-PGK-Blast

Plasmid

#173219

Purpose

piggyBac transposon containing PE2 and Blasticidin expression cassettes

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

PE2
Blast

Use

Piggybac

Expression

Mammalian

Promoter

CMV and hPGK

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pXAT2

Plasmid

#80494

Purpose

AAVS1 sgRNA expression vector

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 sgRNA-T2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9-KRAB

Plasmid

#50917

Purpose

Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

TRE

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9-VP64

Plasmid

#50916

Purpose

Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A nuclease-deficient

Promoter

TRE

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Tags

His and c-myc NLS

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDACR3882 [rab-3p::HYlight::let-858utr]

Plasmid

#207666

Purpose

Expresses worm-optimized HYlight in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight Biosensor

Expression

Worm

Promoter

rab-3

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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