Addgene

We narrowed to 2,378 results for: gcg

Showing: 1081 - 1110 of 2378 results

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pJMP1183

Plasmid

#119254

Purpose

rfp "test" strain

Depositor

Carol Gross, Oren Rosenberg, Jason Peters

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYDE007

Plasmid

#194152

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

Plasmid

#129708

Purpose

Express cre-dependent KASH-EGFP and scramble shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA-his-HYB

Plasmid

#64333

Purpose

his3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of his3 deletion gRNA cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Luciferase shRNA

Plasmid

#225343

Purpose

shRNAmir backbone for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

Luciferase shRNA (LOC116160065 Mouse)

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFUChW Parkin shRNA Mul1 shRNA

Plasmid

#78523

Purpose

Lentiviral vector encoding two shRNAs and mCherry reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1333

Plasmid

#119268

Purpose

rfp "test" strain

Depositor

Carol Gross, Oren Rosenberg, Jason Peters

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_C

Plasmid

#72622

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Rory Johnson, Roderic Guigo

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgMTHFD1L_1

Plasmid

#106316

Purpose

Express Cas9 and sgRNA targeting MTHFD1L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD1L

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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shCDK4/6(2)

Plasmid

#73555

Purpose

vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pJMP1187

Plasmid

#119256

Purpose

rfp "test" strain

Depositor

Carol Gross, Oren Rosenberg, Jason Peters

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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shLacZ

Plasmid

#42559

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shLacZ

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-SETin-FLAG-HA

Plasmid

#24999

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations introduced to prevent siRNA targ…

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2 mBAX

Plasmid

#129577

Purpose

CRISPR deletion of murine BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAX (Bax Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1104

Plasmid

#119248

Purpose

knockdown pqsC in P. aeruginosa

Depositor

Carol Gross, Oren Rosenberg, Jason Peters

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA pqsC (P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LEP-shREN

Plasmid

#105580

Purpose

retrovirally express control shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP1221

Plasmid

#119260

Purpose

rfp "test" strain

Depositor

Carol Gross, Oren Rosenberg, Jason Peters

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKbeta

Plasmid

#26209

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK beta (IKBKB Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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plko.1/shMCT4 #474

Plasmid

#99597

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_TFRC_B

Plasmid

#72626

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Rory Johnson, Roderic Guigo

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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LEP-shMLL-AF9.1039

Plasmid

#105566

Purpose

retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

shRNA targeting MLL part of MLL-AF9 fusion

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKalpha

Plasmid

#26208

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK alpha (CHUK Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
LEP-shMLL-AF9.643

Plasmid

#105565

Purpose

retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

shRNA targeting MLL part of MLL-AF9 fusion

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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