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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,322 results for: pET28a

Showing: 1081 - 1110 of 1322 results

  3. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

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  4. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

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  5. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

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  6. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

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  22. SpyTag-β-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Article
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 β-Lactamase
    Use
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52656-plasmid-map-sequence-id-422741

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  26. SpyTag003-MBP

    Plasmid
    #133450
    Purpose
    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    SpyTag003-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133450-plasmid-map-sequence-id-267039

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  27. SENP2M497A

    Plasmid
    #61088
    Purpose
    cleave the SUMO off when use PETSUMO vector for express fusion protein
    Depositor
    Cynthia Wolberger
    Article
    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
    Insert
    SENP2M497A catalytic domain (SENP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M497A; Catalytic domain is residues 364-589
    Promoter
    T7
    Available Since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1081 - 1110 of 1322 results