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pFR-mTq2-antiFRET-P2A-K-GECO1

Plasmid

#191469

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

K-GECO1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-R-GECO1

Plasmid

#191471

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

R-GECO1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:bleach.3

Plasmid

#191925

Purpose

Encodes a GFP design, sf:bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.3

Plasmid

#191914

Purpose

Encodes a GFP design, bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.12

Plasmid

#191913

Purpose

Encodes a GFP design, mid.12

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.12

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72V Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 em-blue-shift.2

Plasmid

#191901

Purpose

Encodes a GFP design, em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

em-blue-shift.2

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.5

Plasmid

#191903

Purpose

Encodes a GFP design, acid.5

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.5

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145M T167V H181V

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.4

Plasmid

#191898

Purpose

Encodes a GFP design, acid.4

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.4

Tags

His Tag

Expression

Bacterial

Mutation

V61L Q69L Y145M L220V

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.3

Plasmid

#191897

Purpose

Encodes a GFP design, acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.2

Plasmid

#191894

Purpose

Encodes a GFP design, slow.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.2

Tags

His Tag

Expression

Bacterial

Mutation

V68M Q69L S72T V112I Y145M T167V L220V V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.10

Plasmid

#191934

Purpose

Encodes a GFP design, sf:mid.10

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.10

Tags

His Tag

Expression

Bacterial

Mutation

L42V Q69L S72A Y145F T167I L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.1

Plasmid

#191885

Purpose

Encodes a GFP design, bleach.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.1

Tags

His Tag

Expression

Bacterial

Mutation

T108L Y145M T167V L220V

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.3

Plasmid

#191884

Purpose

Encodes a GFP design, mid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A T108V Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 thermo.9

Plasmid

#191882

Purpose

Encodes a GFP design, thermo.9

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

thermo.9

Tags

His Tag

Expression

Bacterial

Mutation

T167V T203H E222Q

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 basic.3

Plasmid

#191881

Purpose

Encodes a GFP design, basic.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S V68A S72A Y145S L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.2

Plasmid

#191880

Purpose

Encodes a GFP design, mid.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.2

Tags

His Tag

Expression

Bacterial

Mutation

T108I Y145I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.6

Plasmid

#191879

Purpose

Encodes a GFP design, bleach.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.6

Tags

His Tag

Expression

Bacterial

Mutation

V61L S72C Y145M H181L V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.3

Plasmid

#191877

Purpose

Encodes a GFP design, slow.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.3

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S T167V L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 basic.2

Plasmid

#191911

Purpose

Encodes a GFP design, basic.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.2

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145V L220V V224I

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-CH-GECO2.1

Plasmid

#191470

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

CH-GECO2.1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_ReBphP-PCM_IRES_sfGFP

Plasmid

#166917

Purpose

Express ReBphP-PCM and sfGFP

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

ReBphP-PCM
sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTCW018

Plasmid

#83531

Purpose

Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pARO9-mf_2-pFUS1J2-ARO7i-CYC1t

Expression

Yeast

Promoter

pARO9/pFUS1J2

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pVRb42_up4062

Plasmid

#49727

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf42_up4062

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb41_up1141

Plasmid

#49726

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf41_up1141

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb39_up1413

Plasmid

#49725

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf39_up1413

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb38_up1322

Plasmid

#49724

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf38_up1322

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb33_375

Plasmid

#49723

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf33_375

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRb31_34

Plasmid

#49721

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf31_34

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb30_2079

Plasmid

#49720

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf30_2079

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge