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We narrowed to 7,122 results for: ef1a

Showing: 1801 - 1850 of 7122 results

  1. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

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  2. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113668-plasmid-map-sequence-id-220922

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  6. HIVGKO

    Plasmid
    #112234
    Purpose
    HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a
    Depositor
    Eric Verdin
    Article
    Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10
    Insert
    csGFP-EF1a-mKO2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112234-plasmid-map-sequence-id-448184

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  14. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  15. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  17. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  18. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  19. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

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  26. AYBEv0.1

    Plasmid
    #193966
    Purpose
    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, EF1a, CBh
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193966-plasmid-map-sequence-id-384658

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  30. pDY0973-RADARS iCaspase

    Plasmid
    #182539
    Purpose
    Express RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNA
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Insert
    iCaspase9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182539-plasmid-map-sequence-id-356646

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  31. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  32. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  33. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  34. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  35. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

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  39. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  43. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    7xTCF-LEF and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  44. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, Chicken, S. pyogenes)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  45. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Mutation
    Promoter
    4xHRE_YB TATA and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  46. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  48. pMK1254

    Plasmid
    #133057
    Purpose
    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.
    Depositor
    Martin Kampmann
    Article
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133057-plasmid-map-sequence-id-267979

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  49. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210706-plasmid-map-sequence-id-416266

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  50. pMK1253

    Plasmid
    #133058
    Purpose
    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.
    Depositor
    Martin Kampmann
    Article
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mClover2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133058-plasmid-map-sequence-id-267978

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Showing: 1801 - 1850 of 7122 results