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Showing: 1801 - 1850 of 6924 results

  1. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  2. gGBEv6.3-SpG

    Plasmid
    #202630
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpG(D10A)-MPGv6.3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    hU6, EF1a, CBh
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202630-plasmid-map-sequence-id-398429

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  6. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  7. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-440858

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  8. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

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  9. CMV-AausFP1-Z1

    Plasmid
    #135048
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    AausFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135048-plasmid-map-sequence-id-269407

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  10. pLV-Blast_hCOL4A5-HA

    Plasmid
    #198103
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198103-plasmid-map-sequence-id-389259

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  11. Cofilin1_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64370
    Purpose
    lentiviral expression of Cofilin1 and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Cofilin1 (CFL1 Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. MLCK_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64369
    Purpose
    lentiviral expression of Myosin Light Chain Kinase and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Myosin Light Chain Kinase (MYLK Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pLV-Blast_hCOL4A5-HA_C1567R

    Plasmid
    #198105
    Purpose
    lentiviral expression of hCOL4A5_C1567R in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    C1567R
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198105-plasmid-map-sequence-id-389261

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  14. pLV-Blast_hCOL4A5-HA_G869R

    Plasmid
    #198104
    Purpose
    lentiviral expression of hCOL4A5_G869R in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    G869R
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198104-plasmid-map-sequence-id-389260

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  15. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

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  17. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  18. Limk1_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64368
    Purpose
    lentiviral expression of Link1 and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Limk1 (LIMK1 Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219821-plasmid-map-sequence-id-440859

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  20. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  22. pLV Erh-V5

    Plasmid
    #175123
    Purpose
    Lentiviral expression of mouse Erh-V5
    Depositor
    Larry Gerace
    Insert
    Erh (Erh Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175123-plasmid-map-sequence-id-351935

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  23. Aiolos in Artichoke

    Plasmid
    #74452
    Purpose
    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    74452-plasmid-map-sequence-id-140673

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  24. pLV V5-lamin B2

    Plasmid
    #175109
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb2
    Depositor
    Larry Gerace
    Insert
    Lmnb2 (Lmnb2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175109-plasmid-map-sequence-id-351741

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  25. pLV_Hygro_mini-mAgrin-Myc-His

    Plasmid
    #198130
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Lentiviral
    Tags
    TEV-Myc-His
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198130-plasmid-map-sequence-id-395039

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  26. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  27. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

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  28. ELL1 Streptag

    Plasmid
    #127278
    Purpose
    Expresses mouse ELL1 with streptactin tag in mammalian cells
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127278-plasmid-map-sequence-id-250019

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  29. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  30. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  31. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

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  33. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

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  34. pEF-GalNAcT2-moxGFP2

    Plasmid
    #218966
    Purpose
    Expresses full length GalNAcT2-moxGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-moxGFP2 (GALNT2 Human)
    Use
    Tags
    moxGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218966-plasmid-map-sequence-id-445673

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  35. pAAV hSyn-Flex RG-cerulean

    Plasmid
    #98221
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV
    Tags
    cerulean
    Expression
    Mutation
    Promoter
    human Synapsin
    Available since
    Aug. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98221-plasmid-map-sequence-id-192715

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  36. pLV Agpat2-V5

    Plasmid
    #175159
    Purpose
    Lentiviral expression of mouse Agpat2-V5
    Depositor
    Larry Gerace
    Insert
    Agpat2 (Agpat2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175159-plasmid-map-sequence-id-352271

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  37. pLV Cnep1r1-V5

    Plasmid
    #175133
    Purpose
    Lentiviral expression of mouse Cnep1r1-V5
    Depositor
    Larry Gerace
    Insert
    Cnep1r1 (Cnep1r1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175133-plasmid-map-sequence-id-352020

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  38. pLV H2afy-V5

    Plasmid
    #175122
    Purpose
    Lentiviral expression of mouse Macroh2a1-V5
    Depositor
    Larry Gerace
    Insert
    Macroh2a1 (Macroh2a1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175122-plasmid-map-sequence-id-351930

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  39. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  40. pEF-GalNAcT2-mGold

    Plasmid
    #218964
    Purpose
    Expresses full length GalNAcT2-mGold in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-mGold (GALNT2 Human)
    Use
    Tags
    mGold
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218964-plasmid-map-sequence-id-445690

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  41. pEF-GalNAcT2-msYFP2

    Plasmid
    #218963
    Purpose
    Expresses full length GalNAcT2-msYFP2 in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msYFP2 (GALNT2 Human)
    Use
    Tags
    msYFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218963-plasmid-map-sequence-id-446179

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  42. pEF-GalNAcT2-msGFP2

    Plasmid
    #218965
    Purpose
    Expresses full length GalNAcT2-msGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msGFP2 (GALNT2 Human)
    Use
    Tags
    msGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218965-plasmid-map-sequence-id-446216

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  43. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  44. pLV V5-Eva1b

    Plasmid
    #175158
    Purpose
    Lentiviral expression of V5-tagged mouse Eva1b
    Depositor
    Larry Gerace
    Insert
    Eva1b (Eva1b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175158-plasmid-map-sequence-id-352269

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  45. pLV Cenpv-V5

    Plasmid
    #175125
    Purpose
    Lentiviral expression of mouse Cenpv-V5
    Depositor
    Larry Gerace
    Insert
    Cenpv (Cenpv Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175125-plasmid-map-sequence-id-351968

    Enlarge

  46. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125196-plasmid-map-sequence-id-243601

    Enlarge

  50. pKAM-E-Flag-GFP

    Plasmid
    #101866
    Purpose
    Lentiviral Evi-1-Flag with GFP marker
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    101866-plasmid-map-sequence-id-211119

    Enlarge

Showing: 1801 - 1850 of 6924 results