Addgene

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pHcPro (GB0067)

Plasmid

#68196

Purpose

Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

TEV HcPro coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pIS47 C20orf139 3'UTR mut

Plasmid

#14490

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR mutant (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

Plasmid

#234881

Purpose

L1HS-silencing plasmid (CRISPRi gRNA2)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

LacZ gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-eGFP-SAPAP3

Plasmid

#176853

Purpose

Expresses SAPAP3 protein fused with eGFP at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

eGFP-SAPAP3

Use

AAV

Tags

GFP

Expression

Bacterial

Mutation

Promoter

hSyn1

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-eGFP-SAPAP3

Plasmid

#176859

Purpose

Expresses SAPAP3 protein fused with eGFP at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

eGFP-SAPAP3

Use

AAV

Tags

GFP

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pOPO812

Plasmid

#195306

Purpose

pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose inducible arapBAD

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pOPO646

Plasmid

#195307

Purpose

pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

TnsD of McCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose inducible arapBAD

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCASCADE-xylA

Plasmid

#158611

Purpose

Low phosphate inducible gRNA silencing the xylA promoter

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-udhA-xylA

Plasmid

#158612

Purpose

Low phosphate inducible gRNA array to silence the xylA and udhA promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

udhA-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA2-xylA

Plasmid

#158613

Purpose

Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

gltA2-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-zwf-xylA

Plasmid

#158614

Purpose

Low phosphate inducible gRNA array to silence the xylA and zwf promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

zwf-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMB204: UbC-GFP-Tg_SAP30L-WPRE

Plasmid

#168109

Purpose

Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

SAP30L (SAP30L T. guttata)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

UbC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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IDA3::HA exon2 (WS13)

Plasmid

#126874

Purpose

p3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen N-term (WS14)

Plasmid

#126875

Purpose

NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

NeonGreen

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen exon2 (WS15)

Plasmid

#126876

Purpose

NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanogHD-pgk-hph

Plasmid

#74909

Purpose

Mammalian expression of mNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha1_SF (GB0106)

Plasmid

#68232

Purpose

Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTAct2 (GB0210)

Plasmid

#68191

Purpose

Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPMtb (GB0080)

Plasmid

#68167

Purpose

Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlMtb promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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