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pFIN-mIRBP156-CHER-RK-GFP-WPRE

Plasmid

#44593

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

IRBP156-CHER
RK-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human rhodopsin kinase (pos 3723-4019) and murine…

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe human Bmf

Plasmid

#17240

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pBEST-p70b-UTR1-deGFP-6xHis-T500

Plasmid

#92222

Purpose

Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70b (p70a promoter with one mutation)

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-PTEN-deltaPDZ

Plasmid

#110182

Purpose

Expression of a mutated PTEN lacking its 4 last aa, and tagged with EGFP

Depositor

Jose Esteban

Article

Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

Insert

PTEN (Pten Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of last 4 amino acids

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-PGK-Puro

Plasmid

#110842

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-Puro

Plasmid

#110838

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-FNLS

Plasmid

#110836

Purpose

CMV expression vector for BE3-FNLS construct (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-hCGN

Plasmid

#236507

Purpose

For expression of Cingulin (human) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (CGN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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hCGN-HA

Plasmid

#236508

Purpose

For expression of Cingulin (human) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (CGN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLC025 - pL0_StopCodon (CT)

Plasmid

#203905

Purpose

MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

Stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN381 - pTRV2-CHLH

Plasmid

#203886

Purpose

Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CHLH fragment

Use

RNAi

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1243 - pL0_pT3O (pro + 5U)

Plasmid

#203891

Purpose

T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3O promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1198 - pL0_pD4H (pro + 5U)

Plasmid

#203894

Purpose

D4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

D4H promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1S scramble

Plasmid

#202837

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 1S scramble control

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

6

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 2S scramble

Plasmid

#202839

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 2S scramble control

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-JAM2-SVA

Plasmid

#202832

Purpose

pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

JAM2 intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-POFUT2-SVA

Plasmid

#202831

Purpose

pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

POFUT2 intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-HTT-SVA

Plasmid

#202833

Purpose

pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

HTT intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-CHAF1B-SVA

Plasmid

#202829

Purpose

pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

CHAF1B intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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