We narrowed to 1,419 results for: RCA

1. HA-VHL Y112N-pBabe-puro

   Plasmid

   #19238

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 112 to Asparagin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-VHL Y112H-pBabe-puro

   Plasmid

   #19237

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 112 to Histidin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSMP-MBD3_3

   Plasmid

   #36373

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MBD3 (MBD3 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

4. ST-SBP-EGFP_puromycin

   Plasmid

   #65270

   Purpose

   ST reporter only ( no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RNA polymerase beta prime subunit FLAG

   Plasmid

   #102337

   Purpose

   Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

   Depositor

   Erin O'Shea

   Article

   Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

   Insert

   RNAP Beta Prime Subunit with C-terminal FLAG TAG
   

   Use

   Tags

   3x FLAG with 3X GSGS linker

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HsSPHK2

   Plasmid

   #118092

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-VHL L188V-pBabe-puro

   Plasmid

   #19241

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Leucin 188 to Valin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LifeAct-TagBFP-DiB-RM

   Plasmid

   #168782

   Purpose

   Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

   Depositor

   Jens Meiler

   Article

   Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

   Insert

   LifeAct-TagBFP-DiB-RM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Str-KDEL_ManII-SBP-tagBFP

   Plasmid

   #65254

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pG4-E6s-luc

    Plasmid

    #46324

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    5xGal4
    

    Use

    Luciferase

    Tags

    E boxes and luciferase

    Expression

    Mammalian

    Mutation

    five copies of a multimerized GAL4 (5×GAL4) site …

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

11. HA-VHL Y98H-pBabe-puro

    Plasmid

    #19235

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Histidin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-VHL 1-115-pBabe-puro

    Plasmid

    #19243

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 116 - 213 (end)

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL1

    Plasmid

    #48741

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to -141)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    truncated promoter region (see pGL6)

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSMP-MBD2_2

    Plasmid

    #36369

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD2 (MBD2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pSMP-MBD2_1

    Plasmid

    #36368

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD2 (MBD2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pTre2tet)::Fbxl21

    Plasmid

    #51890

    Purpose

    expression of Fbxl21

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Fbxl21 (Fbxl21 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV tet

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAC-luc-TimRA

    Plasmid

    #33133

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

    Insert

    Tim-RA 3' UTR (tim Fly)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. HA-VHL T157I-pBabe-puro

    Plasmid

    #19240

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Threonin 157 to Isoleucin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-VHL A149T-pBabe-puro

    Plasmid

    #19242

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Alanin 149 to Threonin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL5

    Plasmid

    #48745

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to +2064)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    truncated promoter region (see pGL6)

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only