We narrowed to 1,028 results for: biotin
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pRibo-Sec-APEX2
Plasmid#111699PurposeTheophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)DepositorInsertAPEX2
TagsV5ExpressionBacterialPromoterhsp60-riboAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pTCF79 H-2D(b) (CD8 mut)
Plasmid#180453PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b) (CD8 mut)
TagsBSP41ExpressionBacterialMutationD227K, replacement of transmembrane domain with e…Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-HIS3
Plasmid#229230PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-PM-miniTurbo-V5_IDG-K
Plasmid#214454PurposeGateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPM-miniTurbo-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
D92_Ld_360170
Plasmid#135905PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserthypothetical protein, conserved
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_NLS-Halo-eGFP-BioID
Plasmid#218448Purposeexpresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BirA
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a Bm-iPGM-bio
Plasmid#162565PurposeExpresses B. malayi iPGM containing a C-terminal biotinylation site and His tagDepositorInsertB. malayi iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
D44_Ld_180090
Plasmid#135858PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertalpha glucosidase II subunit, putative
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT4
Plasmid#223332PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
Tags3HA-miniTurboID-EGFPExpressionPlantPromoterCaMV35SAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ce-iPGM-bio
Plasmid#162559PurposeExpresses C. elegans iPGM containing a C-terminal biotinylation site and His tagDepositorInsertC. elegans iPGM bio-6His (ipgm-1 Nematode)
Tags6His tag, T7 tag, Thrombin cleavage site, and bio…ExpressionBacterialPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
ENDOD1-bio-His
Plasmid#53155PurposeExpresses full-length ENDOD1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertENDOD1 (ENDOD1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD
Plasmid#179407PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBDDepositorAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN1A-c001
Plasmid#175491PurposeProtein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.DepositorAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN2A-c001
Plasmid#175492PurposeProtein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.DepositorAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q)-6xHis
Plasmid#175784PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only
