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Plasmid#18956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
7TFP CDH1 mutant reporter
Plasmid#91703Purposelentiviral luciferase reporter containing CDH1 promoter with mutant E-boxesDepositorInsertCDH1 mutant promoter (CDH1 Human)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationmutant E-boxesPromoterCDH1 mutantAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-RanV19 E1RA2
Plasmid#118317PurposeExpression of human RanDepositorUseTagsGFPExpressionMammalianMutationin-frame insertion of RanV19 into GFPEpac1RA2 and…PromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.BRWD3-V5H
Plasmid#48624PurposeExpresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cellsDepositorInsertBRWD3 (BRWD3 Fly)
UseTagsV5 and His tagsExpressionMammalianMutationno mutations, stop was removed to fuse V5His at C…PromoterCMVAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUN1224 - pL0_pT3R (pro + 5U)
Plasmid#203892PurposeT3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertT3R promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI or BsaI cut site for MoClo domes…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1215 - pL0_pDAT (pro + 5U)
Plasmid#203895PurposeDAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertDAT promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI or BsaI cut site for MoClo domes…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-RB1
Plasmid#87836PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.DepositorInsertsgRB1 (RB1 Human)
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsExpressionMammalianMutationPromoterTight TRE promoter and U6Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pb-miRE-tre-3xFLAG-hnRNPU
Plasmid#196087PurposeExpresses mouse hnRNPU with a 3xFLAG tag in mammalian cellsDepositorInsertSAF-A (Hnrnpu Mouse)
UseTagsN-terminal 3xFLAGExpressionBacterial and MammalianMutationPromoterTREAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneUseTagsGST (glutathione S-transferase)ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-pSnail-L5
Plasmid#186365PurposeEntry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertSnail regulatory sequence
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-pFOGl-L5
Plasmid#186364PurposeEntry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertFriend Of Gata regulatory sequence
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-a-element-L4
Plasmid#186366PurposeEntry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequencesDepositorInserta-element early Otx neural enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
UseTags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7oGFP
Plasmid#69346PurposeOrthogonal GFP message under T7 promoterDepositorInsertSuper folder GFP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10
Plasmid#197952PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspcoCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26 and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L4-UBI-L1R
Plasmid#45959DepositorInserthuman UBI promoter (UBC Synthetic, Human)
UsePentr plasmidTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLI_TNF
Plasmid#171179Purposeinducible expression of human TNFDepositorInserthuman TNF
UseLentiviralTagsnoneExpressionMammalianMutationPromotertight TRE promoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only