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Showing: 721 - 740 of 830 results

  2. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Use
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7/CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  3. pOD2087-trnDEG

    Plasmid
    #89369
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmec-18
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89369-plasmid-map-sequence-id-177235

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  10. pVITRO1-HAPPID1

    Plasmid
    #204626
    Purpose
    Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.
    Depositor
    James McDonnell
    Article
    Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75
    Inserts
    HAPPID1 heavy chain
    HAPPID1 light chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse elongation factor 1 alpha and rat elongatio…
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204626-plasmid-map-sequence-id-416168

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  11. pQCXIP-SHMY-A1Pi

    Plasmid
    #182648
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    A1Pi (SERPINA1 Human)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182648-plasmid-map-sequence-id-358630

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  12. pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

    Plasmid
    #205283
    Purpose
    GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)
    Depositor
    Matthias Prigge
    Insert
    GCaMP8f
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205283-plasmid-map-sequence-id-412570

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  15. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid
    #158133
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-TetRVP64
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    158133-plasmid-map-sequence-id-309383

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  16. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid
    #158135
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-Gal4VP64
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158135-plasmid-map-sequence-id-309380

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  17. pOGS539_rPD-L1nb

    Plasmid
    #226717
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)
    Depositor
    Gautam Dantas
    Article
    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2
    Insert
    Recombinant PD-L1 nanobody
    Use
    Tags
    HA tag, Histidine tag, and Mating factor alpha se…
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226717-plasmid-map-sequence-id-455449

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  18. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    216337-plasmid-map-sequence-id-456751

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  20. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid
    #79649
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79649-plasmid-map-sequence-id-175067

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Showing: 721 - 740 of 830 results