We narrowed to 2,413 results for: organization Addgene

1. pBS CMV NA 3C FLAG SA

   Plasmid

   #234993

   Purpose

   Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NA
   

   Use

   Tags

   HRV 3C site, FLAG, Strep-Tactin (SA)

   Expression

   Mammalian

   Mutation

   In the Streptavidin coding sequence Glu44Val/Ser4…

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNA 3C myc scfvFLAG

   Plasmid

   #234990

   Purpose

   Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NA-3C-myc-scfvFLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNA 3C myc scfvHA

   Plasmid

   #234991

   Purpose

   Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NA-3C-myc-scfvHA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE

   Plasmid

   #225353

   Purpose

   Lentiviral vector for DKK1 and BFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   Dkk1 (Dkk1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

   Plasmid

   #225350

   Purpose

   piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   H2B (H2B Synthetic, Human)
   

   Use

   Tags

   miRFP and non-fluorescent GFP (Y67F)-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3-Ctr1-gRNA-EGFP

   Plasmid

   #226000

   Purpose

   Negative control for CRISPRi-KD

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   negtive control sgRNA of no specific targets in human genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-Ctr2-gRNA-EGFP

   Plasmid

   #226001

   Purpose

   Negative control for CRISPRi-KD

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   negtive control sgRNA of no specific targets in human genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-hL1-gRNA1-EGFP

   Plasmid

   #226002

   Purpose

   CRISPRi-KD of human LINE1

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   sgRNA targeting human L1HS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-hL1-gRNA2-EGFP

   Plasmid

   #226003

   Purpose

   CRISPRi-KD of human LINE1

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   sgRNA targeting human L1HS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5 Myc-tagged PSK (K57A)

    Plasmid

    #197150

    Purpose

    Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST_CAHS1_PARRC_150-189 (pBS0658)

    Plasmid

    #185201

    Purpose

    For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS1_PARRC_150-189
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid

    #185299

    Purpose

    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid

    #185298

    Purpose

    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

    Plasmid

    #185314

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST_APOE_HUMAN_A234G (pBS0816)

    Plasmid

    #185267

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_A234G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A234G

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST_Q19228_CAEEL_93-127 (pBS0735)

    Plasmid

    #185231

    Purpose

    For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    Q19228_CAEEL_93-127
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

    Plasmid

    #185207

    Purpose

    For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    D4NWF2_DrHD_b03_dom3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST_APOA4_ANDDA_21-367 (pBS0742)

    Plasmid

    #185234

    Purpose

    For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_21-367. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOA4_ANDDA_21-367
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST_Q7JLY3_CAEEL_48-124 (pBS0738)

    Plasmid

    #185232

    Purpose

    For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    Q7JLY3_CAEEL_48-124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST_Q19228_CAEEL_51-88 (pBS0734)

    Plasmid

    #185230

    Purpose

    For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-88. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    Q19228_CAEEL_51-88
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge