We narrowed to 2,540 results for: organization Addgene

1. mKate2-P2A-APEX2-2xML1N(7Q)

   Plasmid

   #67666

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1Nmut*2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAHS2parrc-mCh-2xFKBP (pBS1109)

   Plasmid

   #185304

   Purpose

   For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS2parrc
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST_MAHS_RAMVA (pBS0310)

   Plasmid

   #185169

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA
   

   Expression

   Mammalian

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. P1_Firre_1.49_pDONR221

   Plasmid

   #195204

   Purpose

   Plasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.

   Depositor

   John Rinn

   Article

   Hacisuleyman et al Nat Struct Mol Biol. 2014 Feb;21(2):198-

   Insert

   mFirre 1.49 Clone
   

   Use

   Gateway cloning intermediate

   Mutation

   Only exons

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniSOG-Clathrin-15

   Plasmid

   #57764

   Purpose

   Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Clathrin (CLTB Human)
   

   Tags

   miniSOG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. mCherry-miniSOG-H2B-C-10

   Plasmid

   #55090

   Purpose

   Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. mCherry-miniSOG-EB3-7

   Plasmid

   #55089

   Purpose

   Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   EB3 (MAPRE3 Human)
   

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4

   Plasmid

   #112839

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SH3 (N-term) domain of Grb2

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ApoE2_mCh-SspB (pBS1142)

   Plasmid

   #185324

   Purpose

   For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE2
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. deltaVP1 + VP2C-GFP

    Plasmid

    #166676

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

    Plasmid

    #185328

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_81-236-FLAG
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTTH+mEGFP-LANA-H1.4CTD

    Plasmid

    #157802

    Purpose

    Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA-H1.4CTD (H1-4 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5FRT/Flag-hINO80 HSA (212-526)

    Plasmid

    #29443

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 212-526 (a.a.)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

14. mCherry-miniSOG-Clathrin-15

    Plasmid

    #55088

    Purpose

    Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Clathrin (CLTB Human)
    

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5FRT/Flag-hINO80N1 (1-262)

    Plasmid

    #29441

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 1-262 (a.a.)

    Available Since

    Aug. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

16. miniSOG-EB3-7

    Plasmid

    #57765

    Purpose

    Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. miniSOG-Vimentin-7

    Plasmid

    #57779

    Purpose

    Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Vimentin (VIM Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. mCherry-miniSOG-CDC42-C-10

    Plasmid

    #55087

    Purpose

    Localization: Membrane, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    CDC42 (CDC42 Human)
    

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. miniSOG-Keratin-17

    Plasmid

    #57770

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Keratin (KRT18 Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA5FRT/Flag-hINO80C (1224-1556)

    Plasmid

    #29445

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 1224-1556 (a.a.)

    Available Since

    Aug. 1, 2011

    Availability

    Academic Institutions and Nonprofits only