We narrowed to 7,895 results for: sms
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Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO7A-TAIL
Plasmid#89585PurposeExpression plasmid for a positive control bait protein in NanoSPD 2.0 assays.DepositorInsertMYO7A (Myo7a Mouse)
TagsEGFPExpressionMammalianMutationTruncation encoding amino acids 870-2166 of MYO7A…PromoterCMVAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO10-HMM
Plasmid#87256PurposeExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MYRIP
Plasmid#89586PurposeExpression plasmid for a positive control prey protein in NanoSPD 2.0 assays.DepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIZ-EGFP-MYO10-HMM
Plasmid#87257PurposeExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionInsectMutationMyo10 is truncated to contain aa.1-941PromoterOpIE2Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc V5 dDispatched WT HA
Plasmid#126405PurposeExpression of V5 dDispatched WT HA in Drosophila cells lines.DepositorAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis
Plasmid#156581PurposeMammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertmCherry
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
eTC GFP ß-actin ΔZip
Plasmid#27124DepositorInsertbeta-actin (ACTB Human)
TagsEGFP and TC tagExpressionMammalianMutationThis construct lacks β-actin 3′UTR and therefore …Available SinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis
Plasmid#156773PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC7 (SIGLEC7 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC9-Fc(DAPA)-AviTag-6xHis
Plasmid#156771PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC9 (SIGLEC9 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDH3-PA-GFP
Plasmid#18819DepositorInsertPhotoactivatable Green Fluorescent Protein
UseYeast taggingAvailable SinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD33-Fc(DAPA)-AviTag-6xHis
Plasmid#156694PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD33 (CD33 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis
Plasmid#156543PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPDCD1 (PDCD1 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
DGK zeta
Plasmid#35410DepositorAvailable SinceFeb. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PA-GFP-TRP1
Plasmid#18821DepositorInsertPhotoactivatable Green Fluorescent Protein
UseYeast taggingAvailable SinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only