We narrowed to 6,263 results for: wus

1. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-DEST51-GOLPH3

   Plasmid

   #21690

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Invitrogen gateway destination vector

   Tags

   6xHIS and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcmv3-STING-C1471S-FLAG

   Plasmid

   #198188

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C1471S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The cysteine in STING at position 147 mutates to …

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcmv3-STING-C64S,C65S-FLAG

   Plasmid

   #198186

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The cysteine in STING at position 64/65 mutates t…

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-GOLPH3

   Plasmid

   #21688

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-hSyn-UDP1.0-WPRE

   Plasmid

   #220849

   Purpose

   AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

   Depositor

   Yulong Li

   Article

   Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

   Insert

   UDP1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin (hSyn)

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-GlmU-NahK-ET64

   Plasmid

   #234663

   Purpose

   Expression an ELP-tagged fusion enzyme of GlmU and NahK

   Depositor

   Xianwei Liu

   Article

   Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

   Inserts

   N-acetylglucosamine- 1-phosphate uridyltransferase
   N-acetylhexosamine 1-kinase
   

   Use

   Tags

   ELP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-tmpknot-epegRNA-puro backbone

   Plasmid

   #214089

   Purpose

   Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-pegRNA-puro backbone

   Plasmid

   #214085

   Purpose

   Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

    Plasmid

    #129405

    Purpose

    circular Pepper RNA

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-Pepper(TAR Variant-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GfaABC1D HRP-TM

    Plasmid

    #231499

    Purpose

    Expressing HRP on the cell surface of astrocytes

    Depositor

    Baljit Khakh

    Article

    Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

    Insert

    HRP
    

    Use

    AAV

    Tags

    Igk, HA, Myc, PDGFR-beta transmembrane domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn1 HRP-TM

    Plasmid

    #231500

    Purpose

    Expressing HRP on the cell surface of neurons

    Depositor

    Baljit Khakh

    Article

    Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

    Insert

    HRP
    

    Use

    AAV

    Tags

    Igk, HA, Myc, PDGFR-beta transmembrane domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-CytERM-mCherry-(F30-2xPepper)10

    Plasmid

    #129403

    Purpose

    CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    CytERM-mCherry-(F30-2xPepper)10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-AsCas12f-full-length-sgRNA

    Plasmid

    #204638

    Purpose

    Expression of full-length AsCas12f sgRNA in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    full-length AsCas12f sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p15a-AsCas12f-blaZ

    Plasmid

    #171609

    Purpose

    AsCas12f1-based E. coli genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6-AsCas12f-sgRNA-v2

    Plasmid

    #204639

    Purpose

    Expression of trancated AsCas12f sgRNA in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    trancated AsCas12f sgRNA (sgRNA-v2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. psgRNAv1-empty

    Plasmid

    #171611

    Purpose

    sgRNA_v1 plasmid for AsCas12f1 in bacteria

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1_sgRNA_1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Rab7Q67L

    Plasmid

    #169038

    Purpose

    Express human constitutively active form of Rab7a(Q67L) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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