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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-eBE-Y130F

Plasmid

#113423

Purpose

Expresses hA3A-eBE-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGE013

Plasmid

#108681

Purpose

All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

Depositor

Takayuki Kohchi, Shigeo Sugano

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 SIRT1-FL

Plasmid

#105670

Purpose

Mammalian expression construct of mouse SIRT1 full length isoform

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-eYFP

Plasmid

#104056

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP

Use

AAV

Promoter

TRE

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Mito-Car-GECO

Plasmid

#100765

Purpose

Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-Mito-Car-GECO

Use

Lentiviral

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR-S12

Plasmid

#84031

Purpose

To episomally express codon optimized Cas9 and chimeric guide RNA

Depositor

Sen Wu

Article

Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

Inserts

hSpCas9
eGFP

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV and CMV (downstream of F2A self-cleaving pept…

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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U6.3>Control.gRNA.f+e

Plasmid

#99140

Purpose

Control gRNA under the regulation of an optimized chicken specific U6 promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

Control gRNA

Use

CRISPR

Promoter

Chicken U6.3

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pMThy1.1_hDDX5_FL

Plasmid

#88873

Purpose

constitutive expression of DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_WT

Plasmid

#82888

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMyBADC-kan

Plasmid

#84689

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

AraC

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK DN Rac1

Plasmid

#84642

Purpose

Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Dominant Negative

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pTag-RFP-C-h-Rab11a

Plasmid

#79806

Purpose

Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_3

Plasmid

#78200

Purpose

Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A17C,I76G,A197C)

Expression

Bacterial

Mutation

A17C,I76G,A197C (please see depositor comment bel…

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBAtC

Plasmid

#78097

Purpose

Plant expression of Cas9 and gRNA empty backbone; Basta resistance

Depositor

Jin-Soo Kim

Article

Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

NLS-HA

Expression

Plant

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SLC38A9.1

Plasmid

#71869

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pDEST-mCherry-LacR-PALB2

Plasmid

#71112

Purpose

mammalian expession of mCherry-LacR-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTM1_NP_ZEBOV

Plasmid

#69121

Purpose

Encodes for Ebola Virus Zaire NP under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

NP ZEBOV (NP )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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