We narrowed to 16,131 results for: form

1. p3XFlag-CARMIL2

   Plasmid

   #118740

   Purpose

   To express CARMIL2 with Flag tag on N-terminus

   Depositor

   John Cooper

   Article

   Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

   Insert

   CARMIL2 (CARMIL2 Human)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 GTF2E1

   Plasmid

   #67089

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2E1 (GTF2E1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 EBNA1BP2

   Plasmid

   #67072

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. IX301: pMVP (L3-L2) myc epitope tag + polyA

   Plasmid

   #121753

   Purpose

   pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   myc epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. attB‐mCh­‐Poly(A)-­PuroR

   Plasmid

   #65529

   Purpose

   PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-mCherry
   

   Use

   Mouse Targeting; Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LN-entry

   Plasmid

   #128012

   Purpose

   Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag
   

   Use

   Lamdan entry vector

   Tags

   LN-3xFlag

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cntn4.2-AP-His

   Plasmid

   #71942

   Purpose

   Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Cntn4.2 (Cntn4 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C2_3X Flag

   Plasmid

   #194161

   Purpose

   Expresses eGFP fluorescent protein with a 3X flag tag

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   GFP with 3X Flag
   

   Tags

   3x Flag

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FB027

   Plasmid

   #119712

   Purpose

   Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
   

   Use

   Synthetic Biology

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 GABARAP no-stop

    Plasmid

    #123209

    Purpose

    Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 LC3C no-stop

    Plasmid

    #123208

    Purpose

    Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #104984

    Purpose

    Expresses ST-Renilla reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Tags

    24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL Blast H-Ras G12V E37G

    Plasmid

    #12277

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V E37G (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The E37G mutat…

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pEGFPC1-Intu

    Plasmid

    #65430

    Purpose

    For mammalian expression of EGFP-mouse Inturned

    Depositor

    Aimin Liu

    Article

    Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

    Insert

    Inturned (Intu Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL Blast H-Ras G12V Y40C

    Plasmid

    #12279

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

16. RCAS(A) dRhoA N19 (CT#847)

    Plasmid

    #14002

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    RhoA (Rho1 Fly)
    

    Use

    Retroviral; Avian expression

    Mutation

    T19N

    Available Since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid

    #121751

    Purpose

    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FLAG epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ntn5.a-Fc-His

    Plasmid

    #72107

    Purpose

    Expresses the entire Netrin 5, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn5.a (Ntn5 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223 GABARAPL1 G116A

    Plasmid

    #123084

    Purpose

    Gateway entry clone encoding human GABARAPL1 G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 116 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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