Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,964 results for: T7

Clear keywords
Showing: 7441 - 7460 of 10964 results

  1. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid
    #182305
    Purpose
    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.
    Depositor
    Andrew York
    Article
    Seidel et al (2021), doi: 10.5281/zenodo.5810930
    Insert
    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    Tags
    A modified TorA periplasmic targeting sequence pl…
    Expression
    Bacterial
    Mutation
    Spontaneous mutation of calmodulin's penulti…
    Promoter
    t7
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    182305-plasmid-map-sequence-id-360536

    Enlarge

  9. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

    Plasmid
    #105638
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Champ1 (CHAMP1 Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105638-plasmid-map-sequence-id-203917

    Enlarge

  11. NmMetQ

    Plasmid
    #172111
    Purpose
    Expression of Methionine Binding Protein (MetQ) with signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine
    Promoter
    T7 Promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172111-plasmid-map-sequence-id-344824

    Enlarge

  12. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

    Enlarge

  17. pENTR-D-kcnj15

    Plasmid
    #173145
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj15 (kcnj15 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173145-plasmid-map-sequence-id-343321

    Enlarge

  19. pDONR221-kcnj3a

    Plasmid
    #173129
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj3a (kcnj3a Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173129-plasmid-map-sequence-id-342882

    Enlarge

  20. pDONR221-kcnj5

    Plasmid
    #173132
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj5 (kcnj5 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173132-plasmid-map-sequence-id-343129

    Enlarge

Showing: 7441 - 7460 of 10964 results