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pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Publication

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
dSaCas9-VPR

Plasmid

#205251

Purpose

In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Depositor

Rasmus Bak

Publication

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSaCas9-VPR

Use

CRISPR

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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B4-1

Plasmid

#111475

Purpose

Ala-tRNA synthetase

Depositor

Cheemeng Tan

Publication

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ala-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B1-2

Plasmid

#111464

Purpose

Met-tRNA synthetase

Depositor

Cheemeng Tan

Publication

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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R5 PV

Plasmid

#51734

Purpose

Dicistronic vector with PV IRES

Depositor

Vincent Mauro

Publication

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

PV IRES

Promoter

SV40

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pROP-IP-GFP

Plasmid

#122136

Purpose

GFP Reporter plasmid for B-Rex contain imperfect operator sequence

Depositor

Srivatsan Raman

Publication

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

GFP

Expression

Bacterial

Promoter

J23101

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO iRFP670

Plasmid

#68443

Purpose

TOPO construct expressing iRFP670 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

iRFP670

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Mumps V-FLAG

Plasmid

#44908

Depositor

Curt Horvath

Publication

Ulane et al J Virol. 2003 Jun;77(11):6385-93.

Insert

Mumps V (V/P )

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223 SARS-CoV-2 NSP10_nostop

Plasmid

#149313

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP4_nostop

Plasmid

#149307

Purpose

Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMT-myl7-cytoBirA-2A-mCherry_Ras

Plasmid

#80060

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSico-shRaptor

Plasmid

#81086

Purpose

conditional expression of an shRNA targeting murine Raptor

Depositor

Angelique Bordey

Publication

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Raptor shRNA (Rptor Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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SRGN-bio-His

Plasmid

#52088

Purpose

Expresses full-length Serglycin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SRGN (SRGN Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Publication

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV5 Flag Jnk1a1(APY)

Plasmid

#15742

Depositor

Roger Davis

Publication

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(TPF) (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Mutation

Thr-190Ala

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-NLS-M-MLV-RT

Plasmid

#181804

Purpose

pET28a-NLS-M-MLV RT

Depositor

Erik Sontheimer

Publication

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

NLS-M-MLV-RT

Expression

Bacterial

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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p15aC-GFP

Plasmid

#107862

Purpose

Control plasmid expressing GFP

Depositor

Sanford Simon

Publication

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

GFP

Tags

myc

Expression

Bacterial

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO mTagBFP2

Plasmid

#68413

Purpose

TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTagBFP2

Use

Gmap

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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