We narrowed to 14,584 results for: pET

1. pET15b-His6-CUbo(K48R)-GFP

   Plasmid

   #212721

   Purpose

   Bacterial expression of His6-CUbo(K48R)-GFP

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   GFP
   

   Use

   Tags

   6xHis-CUbo(K48R)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

   Plasmid

   #208630

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   Δ1~34,Δ215~284

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

   Plasmid

   #208632

   Purpose

   for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 53~77, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

   Plasmid

   #208624

   Purpose

   for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 1~77, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDUET-GST AO7RE ∆238-245

   Plasmid

   #139318

   Purpose

   GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET16b-RPA4/His14 (MSW#949)

   Plasmid

   #208075

   Purpose

   Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

   Depositor

   Marc Wold

   Article

   Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

   Insert

   RPA4, RPA14(His-tagged)
   

   Use

   Tags

   10xHis tag on RPA14

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b+His SUMO Δ37 PPM1H

   Plasmid

   #207334

   Purpose

   Bacterial expression of His SUMO Δ37 PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Δ37

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b+His SUMO Rab10(Q68L)

   Plasmid

   #207342

   Purpose

   Bacterial expression of His SUMO Rab10(Q68L)

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   Rab10 (RAB10 Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Q68L

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-B-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207388

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A, amino acids 10…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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