We narrowed to 10,431 results for: plasmids 101

1. pMXs-hs-CDC34

   Plasmid

   #52731

   Purpose

   retroviral expression of human CDC34

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   CDC34 (CDC34 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SECURE BE4max(R33A) (pBM608)

   Plasmid

   #140251

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-SYN_mEGFP-CREB (S133A)

   Plasmid

   #137010

   Purpose

   AAV backbone, mEGFP-CREB donor for G-CREB with S133A mutation

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   AAV

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Changes Serine 133 into Alanine

   Promoter

   hSynapsin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. gh139

   Plasmid

   #106809

   Purpose

   expression of gRNA targeting EXTL3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   EXTL3 (EXTL3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-ETS2

   Plasmid

   #170685

   Purpose

   doxycycline-inducible overexpression of ETS2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   ETS2 (ETS2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-tetO-Id2

   Plasmid

   #70764

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   inhibitor of DNA binding 2 (Id2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pmiRGLO-miR-124-sponge (pos. CTRL)

   Plasmid

   #117327

   Purpose

   Dual luciferase assay positive control for miR-124 binding

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   miR-124 sponge
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Murine PGK-1

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRGLO-miR-124-sponge_rev(neg. CTRL)

   Plasmid

   #117326

   Purpose

   Dual luciferase assay negative control for miR-124 binding

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   reverse miR-124 sponge
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Murine PGK-1

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Linked Free NES

   Plasmid

   #182485

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Linked Free PcPTS

    Plasmid

    #182486

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-PTS
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA BRCA1 C64Y

    Plasmid

    #139326

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 C64Y using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-ESRRG

    Plasmid

    #170684

    Purpose

    doxycycline-inducible overexpression of ESRRG

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-TetOminiCMV-Klf4

    Plasmid

    #136613

    Purpose

    Dox-inducible lentiviral vector expressing mouse Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV2_hSyn_Phobos_Citrine

    Plasmid

    #98216

    Purpose

    Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

    Plasmid

    #205444

    Purpose

    human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-HLA-E SCT-hCD55
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

    Plasmid

    #203841

    Purpose

    non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    2A-mScarlet-KASH

    Mutation

    G144A, Y145A, G146A

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV2_hSyn_Aurora_Citrine

    Plasmid

    #98217

    Purpose

    Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

    Plasmid

    #184381

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Cas9, two gRNAs targeting dystrophin
    

    Use

    Lentiviral

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA BRCA1 Q1779Stop

    Plasmid

    #139331

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 Q1779Stop using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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